Chlorine Sensitivity of Feline Calicivirus, a Norovirus Surrogate
Chlorine Sensitivity of Feline Calicivirus, a Norovirus Surrogate
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DOI：
10.1128/aem.00083-07
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发表时间：
2007-07
影响因子：
4.4
通讯作者：
H. Urakami;K. Ikarashi;K. Okamoto;Yukari Abe;Tamami Ikarashi;T. Kono;Y. Konagaya;N. Tanaka
中科院分区：
文献类型：
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作者：
H. Urakami;K. Ikarashi;K. Okamoto;Yukari Abe;Tamami Ikarashi;T. Kono;Y. Konagaya;N. Tanaka
摘要研究了诺如病毒替代物猫杯状病毒（FCV）对游离氯的敏感性与需氯量的关系。当用含10 μg/ml游离氯的次氯酸钠溶液处理FCV粗悬浮液时，病毒感染性降低的程度明显取决于反应混合物的体积。FCV对游离氯的表观敏感性随着宿主细胞碎片的减少而增加，这表明必须最大限度地减少氯的需求才能了解病毒的真实敏感性。因此，我们从宿主细胞组分中部分纯化了病毒，并发现用300 ng/ml游离氯处理5分钟，FCV的感染性降低超过log 4.6。
ABSTRACT The sensitivity to free chlorine of feline calicivirus (FCV), a norovirus surrogate, was examined relative to chlorine demand. When a crude suspension of FCV was treated with a sodium hypochlorite solution containing 10 μg/ml free chlorine, the extent of the decrease of viral infectivity clearly depended on the volume of the reaction mixture. The apparent sensitivity of FCV to free chlorine increased with the reduction of host cell debris, indicating that chlorine demand must be minimized to know the true sensitivity of the virus. We therefore partially purified the viruses from the host cell components and found that the infectivity of FCV was reduced by more than log 4.6 by 5 min of treatment with 300 ng/ml free chlorine.