Comparison of the genome Treponema denticola with of the oral pathogen other spirochete genomes
Comparison of the genome Treponema denticola with of the oral pathogen other spirochete genomes
复制标题
DOI：
10.1073/pnas.0307639101
复制
发表时间：
2004-04-13
影响因子：
11.1
通讯作者：
Paulsen, IT
中科院分区：
文献类型：
--
作者：
Seshadri, G;Myers, GSA;Paulsen, IT
我们展示了与牙周病相关的口腔螺旋体密螺旋体（ATCC 35405）完整的2,843,201 bp基因组序列。对齿牙牙霉基因组的分析揭示了介导共聚集、细胞信号传导、应激保护和其他竞争和合作措施的因素，与其在龈下牙菌斑混合物种环境中的致病性质和生活方式一致。与先前测序的螺旋体基因组进行比较，揭示了导致螺旋体生理学差异和相似性以及致病潜力的特定因素。齿状螺旋体基因组的大小比相关梅毒螺旋体的基因组大得多。基因含量的差异似乎可归因于三种现象的结合：基因组减少，谱系特异性扩增和水平基因转移。由于还原进化而丢失的基因似乎主要与代谢和运输有关，而由于谱系特异性扩增而产生的一些基因与各种致病性相互作用有关，而通过水平基因转移获得的基因主要与噬菌体相关或功能未知。
We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphilis-causing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phage-related or of unknown function.