nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans.
nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans.
复制标题
DOI：
--
复制
发表时间：
1999-11
期刊：
影响因子：
4.6
通讯作者：
K. Subramaniam;G. Seydoux
中科院分区：
文献类型：
--
作者：
K. Subramaniam;G. Seydoux
在果蝇中，胚胎模式和原始生殖细胞（PGC）发育需要后决定因素纳米。我们已经在秀丽隐杆线虫中发现了三个基因，它们含有一个假定的锌结合结构域，类似于纳米线虫中发现的结构域，并表明其中两个基因在PGC发育过程中起作用。与纳米果蝇一样，秀丽隐杆线虫no -1和no -2通常不需要PGC命运规范，而是调节PGC发育的特定方面。no -2在原肠胚形成前后通过与P颗粒相关的母体RNA在PGCs中表达，并且是PGCs有效整合到体细胞性腺所必需的。no -1在原肠胚形成后的PGCs中表达，并且需要与no -2一起冗余表达，以防止饥饿动物的PGCs分裂，并在幼虫发育期间维持生殖细胞的活力。在没有no -1和no -2的情况下，生殖细胞在第二幼虫期结束时停止增殖，并以部分依赖于凋亡基因ced-4的方式死亡。我们的研究结果还表明，与果蝇相关的rna结合蛋白与nos-1和nos-2的PGC过程相同。这些研究表明，进化上遥远的生物利用保守因子来调节早期生殖细胞的发育和存活，这些因子包括nanos和pumilio基因家族的成员。
In Drosophila, the posterior determinant nanos is required for embryonic patterning and for primordial germ cell (PGC) development. We have identified three genes in Caenorhabditis elegans that contain a putative zinc-binding domain similar to the one found in nanos, and show that two of these genes function during PGC development. Like Drosophila nanos, C. elegans nos-1 and nos-2 are not generally required for PGC fate specification, but instead regulate specific aspects of PGC development. nos-2 is expressed in PGCs around the time of gastrulation from a maternal RNA associated with P granules, and is required for the efficient incorporation of PGCs into the somatic gonad. nos-1 is expressed in PGCs after gastrulation, and is required redundantly with nos-2 to prevent PGCs from dividing in starved animals and to maintain germ cell viability during larval development. In the absence of nos-1 and nos-2, germ cells cease proliferation at the end of the second larval stage, and die in a manner that is partially dependent on the apoptosis gene ced-4. Our results also indicate that putative RNA-binding proteins related to Drosophila Pumilio are required for the same PGC processes as nos-1 and nos-2. These studies demonstrate that evolutionarily distant organisms utilize conserved factors to regulate early germ cell development and survival, and that these factors include members of the nanos and pumilio gene families.