Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development.
Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development.
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天然和重组赖氨酸脱羧酶免疫对犬牙龈炎发展的影响。
DOI：
10.1016/j.vaccine.2012.08.028
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发表时间：
2012
期刊：
影响因子：
5.5
通讯作者：
Levine,Martin
中科院分区：
文献类型：
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作者：
Peters,JenniferL;DeMars,PaulL;Collins,LindsayM;Stoner,JulieA;Matsumoto,Hiroyuki;Komori,Naoka;Singh,Anil;Feasley,ChristaL;Haddock,JamesA;Levine,Martin
牙周病、牙龈炎症（牙龈炎）和牙周附着丧失（牙周炎）导致牙齿脱落和对慢性炎症的易感性。定期洗牙和清洁牙齿可以控制疾病，但对伴侣动物来说是昂贵的。腐蚀艾肯氏菌在犬口腔中很常见，是赖氨酸脱羧酶（LDC）的来源。在人类牙齿生物膜（菌斑）中，LDC将赖氨酸转化为尸胺，并损害牙龈上皮对细菌的屏障。因此，LDC疫苗接种可延缓牙龈炎的发展。岁的比格犬提供血液样本，并记录体重和临床测量结果（生物膜和牙龈炎）。将犬洁治干净后，皮下注射0.2mg大肠杆菌天然LDC。用从大肠杆菌中纯化的0.2mg重组LDC和2组4只狗。第三组4只犬鼻内免疫。Rehydragel®、Emulsigen®、Polygen ™或Carbigen ™用作佐剂。另外四对狗用每种佐剂单独假免疫（对照）。重复免疫两次，间隔3周，并在另外2周后获得临床测量结果，此时再次刮除牙齿并清洁。然后停止刷牙，饮食从硬食物改为软食物。在1、2、3、4、6和8周后重复临床测量。与假免疫的狗相比，用天然LDC皮下免疫后或用Carbigen ™中的重组LDC鼻内免疫后，牙龈炎在软饮食的所有8周内均有所减轻，但用Emulsigen®中的重组LDC皮下免疫后仅持续8周中的6周（重复测量方差分析）。皮下接种诱导了较强的血清IgG抗体反应，在软食期间下降，而鼻内免疫诱导了较弱的血清IgA抗体反应，没有减少。如果程序优化，重组LDC免疫接种可提供牙龈炎保护。
Periodontal disease, gingival inflammation (gingivitis) and periodontal attachment loss (periodontitis), causes tooth loss and susceptibility to chronic inflammation. Professionally scaling and cleaning the teeth regularly controls the disease, but is expensive in companion animals. Eikenella corrodens is common in canine oral cavities where it is a source of lysine decarboxylase (LDC). In human dental biofilms (plaques), LDC converts lysine to cadaverine and impairs the gingival epithelial barrier to bacteria. LDC vaccination may therefore retard gingivitis development. Year-old beagle dogs provided blood samples, and had weight and clinical measurements (biofilm and gingivitis) recorded. After scaling and cleaning, two dogs were immunized subcutaneously with 0.2mg native LDC from E. corrodens and 2 sets of four dogs with 0.2mg recombinant LDC purified from Escherichia coli. A third set of 4 dogs was immunized intranasally. Rehydragel®, Emulsigen®, Polygen™ or Carbigen™ were used as adjuvant. Four additional pairs of dogs were sham-immunized with each adjuvant alone (controls). Immunizations were repeated twice, 3 weeks apart, and clinical measurements were obtained after another 2 weeks, when the teeth were scaled and cleaned again. Tooth brushing was then stopped and the diet was changed from hard to soft chow. Clinical measurements were repeated after 1, 2, 3, 4, 6 and 8 weeks. Compared with sham-immunized dogs, gingivitis was reduced over all 8 weeks of soft diet after subcutaneous immunization with native LDC, or after intranasal immunization with recombinant LDC in Carbigen™, but for only 6 of the 8 weeks after subcutaneous immunization with recombinant LDC in Emulsigen®(repeated measures ANOVA). Subcutaneous vaccination induced a strong serum IgG antibody response that decreased during the soft diet period, whereas intranasal immunization induced a weak serum IgA antibody response that did not decrease. Immunization with recombinant LDC may provide protection from gingivitis if procedures are optimized.