Effect of a neuroprotective exercise protocol on oxidative state and BDNF levels in the rat hippocampus
Effect of a neuroprotective exercise protocol on oxidative state and BDNF levels in the rat hippocampus
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DOI：
10.1016/j.brainres.2007.10.012
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发表时间：
2008-01-10
期刊：
影响因子：
2.9
通讯作者：
Siqueira, Ionara Rodrigues
中科院分区：
文献类型：
--
作者：
Cechetti, Fernanda;Fochesatto, Cintia;Siqueira, Ionara Rodrigues
每天中等强度的运动（2周的20分钟/天的跑步机训练），这减少了损伤的海马脑片从大鼠提交到体外缺血，没有修改氧化应激参数在海马，也没有脑源性神经营养因子（BDNF）在不同的大脑区域的水平。目的是研究海马氧化状态和/或脑BDNF含量的调制是否参与运动诱导的神经保护。Wistar大鼠每天在跑步机上进行运动，并在最后一次跑步机跑步后约16 h处死。一些几个氧化应激参数进行了测定，特别是自由基水平，大分子损伤，总反应性抗氧化潜力和反应性水平，这代表了总的抗氧化能力，在海马。此外，通过ELISA测量大鼠不同大脑区域（海马、皮质、纹状体和小脑）中的BDNF水平。所使用的运动方案没有影响在海马中研究的任何氧化应激参数，这表明它不会引起显著的氧化应激，也不会诱导细胞抗氧化系统的适应。跑步机训练也没有改变大脑区域中BDNF的含量。考虑到这种运动方案已被证明是神经保护的事实，我们可以推测，BDNF水平和氧化状态可能不直接参与缺血后运动诱导的神经保护机制。(c)2007年由Elsevier B. V.出版。
Daily moderate intensity exercise (2 weeks of 20 min/day of treadmill training), which reduces damage to hippocampal slices from rats submitted to in vitro ischemia, did not modify oxidative stress parameters in the hippocampus nor the brain-derived neurotrophic factor (BDNF) levels in different brain regions. The aim was to investigate whether the modulation of hippocampal oxidative status and/or brain BDNF content is involved in exercise-induced neuroprotection. Wistar rats were submitted to daily exercise in the treadmill and were sacrificed approximately 16 h after the last treadmill running. Some several oxidative stress parameters were determined, specifically the free radical levels, the macromolecule damage, the total reactive antioxidant potential and reactivity levels, which represent the total antioxidant capacity, in the hippocampus. in addition, BDNF levels in different rat cerebral regions (hippocampus, cortex, striatum, and the cerebellum) were measured by ELISA. The used exercise protocol did not affect any oxidative stress parameters studied in the hippocampus, suggesting that it does not cause a significant oxidative stress nor induce adaptations of the cellular antioxidant system. Treadmill training also did not change the BDNF content in brain areas studied. Considering the fact that this exercise protocol have been shown to be neuroprotective, we might speculate that BDNF levels and oxidative status may not be directly involved with the mechanisms of exercise-induced neuroprotection after ischemia. (c) 2007 Published by Elsevier B.V.