Cells-to-CT Kits: Next Generation Gene Expression Analysis Workflows that Eliminate Sample Purification
Cells-to-CT Kits: Next Generation Gene Expression Analysis Workflows that Eliminate Sample Purification
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DOI：
10.1016/j.jala.2010.03.004
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发表时间：
2010-10-01
期刊：
影响因子：
--
通讯作者：
Kephart, Dan
中科院分区：
文献类型：
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作者：
Abruzzese, Ron;Fekete, Richard;Kephart, Dan
正常和患病状态下RNA表达的真实的时间逆转录PCR分析是研究和筛选工作流程中使用的基本技术。然而，RNA样品的传统纯化通常代表显著的工作流程瓶颈，这增加了分析所需的时间和自动化复杂性。T技术是一种结合真实的时间分析设计的工作流程，可完全消除对传统纯化的需求，同时保持与纯化RNA相关的性能。我们证明，在宽范围的样品输入中，结果等同于使用传统方法纯化的RNA，同时在处理自动化液体处理器时提供优势（JALA 2010 15 362-8）。
Real time reverse transcription PCR analysis of RNA expression in normal and diseased states is a fundamental technique used in research and screening workflows However traditional purification of RNA samples often represents a significant workflow bottleneck which increases the time and automation complexity required for analysis We describe the Cells to C-T technology a workflow that was designed in con junction with real time analysis to completely eliminate the need for traditional purification while maintaining performance relative to purified RNA High density sample preparation is accomplished in less than 8 min and is highly amenable to automation We demonstrate that results are equivalent to RNA purified using traditional methods over a broad range of sample input while offering advantages when dealing with automated liquid handlers (JALA 2010 15 362-8)