Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
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DOI：
10.1371/journal.pone.0139200
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kuehn MJ
中科院分区：
文献类型：
--
作者：
Kulp AJ;Sun B;Ai T;Manning AJ;Orench-Rivera N;Schmid AK;Kuehn MJ
革兰氏阴性菌产生外膜囊泡的过程已有充分记录。然而，这些囊泡生物发生背后的机制仍不清楚。这里进行了高通量实验方法和系统规模分析，以确定大肠杆菌突变株（庆应义塾保藏）全基因组敲除文库的囊泡形成值。由此产生的数据集定量地概括了之前观察到的表型，并暗示了囊泡形成过程中的近 150 个新基因。基因功能和生化途径分析表明，截断外膜结构（例如脂多糖和肠细菌共同抗原）的突变会导致囊泡过度形成，而氧化应激反应途径中的突变会导致水平降低。这项研究扩展并完善了有关大肠杆菌外膜囊泡形成所需细胞途径的当前知识。
The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and systems-scale analysis was conducted to determine vesiculation values for the whole genome knockout library of Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively recapitulates previously observed phenotypes and implicates nearly 150 new genes in the process of vesiculation. Gene functional and biochemical pathway analyses suggest that mutations that truncate outer membrane structures such as lipopolysaccharide and enterobacterial common antigen lead to hypervesiculation, whereas mutants in oxidative stress response pathways result in lower levels. This study expands and refines the current knowledge regarding the cellular pathways required for outer membrane vesiculation in E. coli.