Labelling of electroreceptive afferents in a gymnotoid fish by intracellular injection of HRP: The mystery of multiple maps
Labelling of electroreceptive afferents in a gymnotoid fish by intracellular injection of HRP: The mystery of multiple maps
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通过细胞内注射 HRP 标记裸鱼中的电感受传入：多个图谱之谜
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
J. Dye
中科院分区：
文献类型：
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作者：
W. Heiligenberg;J. Dye
通过细胞内注射辣根过氧化物酶（HRP）标记裸形目鱼Eigenmannia的生理学鉴定的初级电感受性传入，以确定其在后侧线叶（PLLL）的终止位点（图1）。对于每个终末野，我们绘制了相关受体孔在体表的位置，发现：1）壶腹单位以躯体位置有序的方式投射到内侧PLLL。2）P型和T型的神经单位以躯体位置有序的方式投射到PLLL的三个独立区域，称为中央-内侧、中央-外侧和外侧（图1和2）。第2-4段）。每个结节单位投影到所有三个地图和P-和T-单位的投影是在躯体定位寄存器。
除了电感受单位，机械感受单位也遇到在前侧线神经节，但其中央的投影被发现以外的PLLL，在前侧线叶（ALLL）和在隆起的颗粒。这一发现与Maler等（1974）提出的中枢投射的形态特异性分离的概念相一致，电感受性传入神经的感受孔越靠近身体的尾端，其胞体越大，神经传导越快（图5、6）。来自身体表面的较远区域的传入神经的较快神经传导最小化与身体表面的广泛分离区域中的同步电事件相关联的尖峰到达的时间差异。
SummaryPhysiologically identified primary electroreceptive afferents in the gymnotiform fish,Eigenmannia, were labelled by intracellular injection of horseradish peroxidase (HRP) in order to determine their termination sites in the posterior lateral line lobe (PLLL) (Fig. 1). For each terminal field we mapped the location of the associated receptor pore on the body surface and found:1)Ampullary units project in a somatotopically ordered manner to the medial PLLL.2)Tuberous units, both P- and T-types, project in a somatotopically ordered manner to three separate regions of the PLLL, called central-medial, central-lateral and lateral (Figs. 2–4). Each tuberous unit projects to all three maps and the projections of P- and T-units are in somatotopic register.
In addition to electroreceptive units, mechanoreceptive units were also encountered in the anterior lateral line nerve ganglion, but their central projections were found outside of the PLLL, in the anterior lateral line lobe (ALLL) and in the eminentia granularis. This finding is in accordance with the notion of modality-specific separation of central projections, forwarded by Maler et al. (1974).Tuberous electroreceptive afferents have larger somata and faster nerve conduction the further their receptor pores are located towards the tail end of the body (Fig. 5, 6). The faster nerve conduction of afferents from more distant regions of the body surface minimizes temporal disparity in the arrival of spikes linked to synchronous electrical events in widely separated regions of the body surface.