Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification
Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification
复制标题
环介导等温扩增后基于金纳米颗粒的目标 DNA 比色检测
DOI：
10.1007/s40242-013-2371-9
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发表时间：
2013-06-01
影响因子：
3.1
通讯作者：
Jin Qin-han
中科院分区：
文献类型：
--
作者：
Zhou Chao;Mu Ying;Jin Qin-han
我们建立了一种快速、简便、无需标记的比色法用于靶DNA的鉴定。它是基于环介导等温扩增（LAMP）。普通金纳米颗粒（AuNPs）用于指示LAMP的发生。将扩增产物与AuNP以优化的比例混合，在该比例下，脱氧核糖核苷酸（dNTP）通过配体-金属相互作用与AuNP结合，从而增强AuNP的稳定性。如果靶DNA被扩增，dNTP的急剧减少导致AuNP的聚集和颜色从红色变为蓝色。该方法的成功在很大程度上取决于溶液的离子强度和dNTPs的初始浓度。与其他等温产品鉴定方法不同，该方法简单，可在仪器不足或甚至缺乏的现场应用。
We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA. It is based on loop-mediated isothermal amplification(LAMP). Plain gold nanoparticles(AuNPs) are used to indicate the occurrence of LAMP. The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides(dNTPs) bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.