EXTENSOR AND FLEXOR PROTOPLASTS FROM SAMANEA PULVINI .1. ISOLATION AND INITIAL CHARACTERIZATION
EXTENSOR AND FLEXOR PROTOPLASTS FROM SAMANEA PULVINI .1. ISOLATION AND INITIAL CHARACTERIZATION
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DOI：
10.1104/pp.76.3.680
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发表时间：
1984-01-01
期刊：
影响因子：
7.4
通讯作者：
SATTER, RL
中科院分区：
文献类型：
--
作者：
GORTON, HL;SATTER, RL
原生质体分离自夜形树S的开放枕的伸肌和屈肌区域。萨曼这两种类型的原生质体在分离过程中经历许多变化。伸肌原生质体在体内是单液泡的，但也有一些变成多液泡的。所有的屈肌原生质体都是单液泡的。在开放的枕中，伸肌细胞比屈肌细胞具有更高的渗透压。然而，两种类型的原生质体可以分离，在消化培养基中使用相同的麦芽糖（0.5 M山梨醇）的最佳产量。在收获或消化过程中可能会发生某些骨组织的渗漏，尤其是伸肌组织。尽管有这些变化，两种类型的原生质体都响应于10 μ M褐球菌素（1.6-1.8毫微当量/106个原生质体/分钟）而挤出质子，表明原生质体是代谢活性的，并且质子转运机制必须至少部分起作用。如果从开放叶枕中分离的原生质体具有封闭叶枕中细胞的特征，则液泡结构和渗透压的变化是预期的。
Protoplasts were isolated from extensor and flexor regions of open pulvini of the nyctinastic tree S. saman. Both types of protoplasts undergo many changes during isolation. Extensor protoplasts are univacuolate in vivo, but some become multivacuolate. All flexor protoplasts are univacuolate. In an open pulvinus, extensor cells have a higher osmotic pressure than flexor cells. However, both types of protoplasts can be isolated with optimal yield using the same osmoticum (0.5 M sorbitol) in the digestion medium. Some leakage of osmoticum probably occurs during harvest or digestion, especially from extensor tissue. Despite these changes, both types of protoplasts extrude protons in response to 10 .mu.M fuscicoccin (1.6-1.8 nanoequivalent/106 protoplasts/min), demonstrating that the protoplasts are metabolically active and that proton transport mechanisms must be at least partially functional. The changes in vacuolar structure and osmotic pressure are as expected if the protoplasts, which are isolated from open pulvini, take on characteristics of cells in a closed pulvinus.