Noninvasive and Direct Monitoring of Erythrocyte Aggregates in Human Retinal Microvasculature Using Adaptive Optics Scanning Laser Ophthalmoscopy
Noninvasive and Direct Monitoring of Erythrocyte Aggregates in Human Retinal Microvasculature Using Adaptive Optics Scanning Laser Ophthalmoscopy
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DOI：
10.1167/iovs.12-11138
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发表时间：
2013-06-01
影响因子：
4.4
通讯作者：
Yoshimura, Nagahisa
中科院分区：
文献类型：
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作者：
Arichika, Shigeta;Uji, Akihito;Yoshimura, Nagahisa
目的.应用自适应光学扫描激光检眼镜（AO-SLO）观察红细胞在角膜旁毛细血管中的聚集情况。从10名健康受试者的一只眼的视神经旁区域获取AO-SLO视频。红细胞聚集体被检测为“暗尾”，其是比血管阴影更暗的区域。在靶毛细血管中测量暗尾的长度，并使用时空图像分析其长度的时间依赖性变化。暗尾延伸率计算为靶毛细管每单位长度的暗尾长度的变化。总体平均暗尾长度为112.1 +/- 36.9 μ m。各监测毛细血管的暗尾随时间延长而延长（P < 0.0001）。暗尾延长率和平均速度分别为0.51 ± 0.37和1.49 ± 0.36 mm/s。AO-SLO可用于无创和直接监测视网膜微血管中的血液动力学，而无需染色剂。红细胞聚集体被检测为暗尾，并在正常受试者的血管旁毛细血管中以时间依赖性的方式延长。监测暗尾的特征对于评估视网膜血流动力学具有很好的潜力。
PURPOSE. To investigate erythrocyte aggregates in parafoveal capillaries by adaptive optics scanning laser ophthalmoscopy (AO-SLO).METHODS. AO-SLO videos were acquired from the parafoveal areas of one eye in 10 healthy subjects. Erythrocyte aggregates were detected as "dark tails" that were darker regions than vessel shadows. The lengths of the dark tails were measured in target capillaries, and their time-dependent changes in length were analyzed using spatiotemporal images. The dark tail elongation rate was calculated as the change of dark tail length per unit length of the target capillary.RESULTS. The overall average dark tail length was 112.1 +/- 36.9 mu m. The dark tail became longer in a time-dependent manner in every monitored capillary (P < 0.0001). The dark tail elongation rate and average velocity were 0.51 +/- 0.37 and 1.49 +/- 0.36 mm/s, respectively.CONCLUSIONS. AO-SLO can be used for noninvasive and direct monitoring of blood dynamics in the retinal microvasculature without dying agents. Erythrocyte aggregates were detected as dark tails and were elongated in a time-dependent manner in the parafoveal capillaries of normal subjects. Monitoring the characteristics of dark tails has promising potential for evaluating retinal hemodynamics.