Bipartite nuclear localization signal of matrin 3 is essential for vertebrate cells
Bipartite nuclear localization signal of matrin 3 is essential for vertebrate cells
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DOI：
10.1016/j.bbrc.2006.12.191
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发表时间：
2007-03-02
影响因子：
3.1
通讯作者：
Kitagawa, Yasuo
中科院分区：
文献类型：
--
作者：
Hisada-Ishii, Shoji;Ebihara, Mizuki;Kitagawa, Yasuo
Matrin 3是一种核基质蛋白，具有以下潜力：（1）与p54（nrb）和PSF合作将混杂编辑的RNA保留在核内，（2）介导NMDA诱导的神经元死亡，以及（3）调节基质/支架附着区（MAR/SAR）附近基因的启动子活性。我们鉴定了鸡母蛋白3（cmatr 3）在残基583-602处的二分核定位信号（NLS）。通过在鸡DT 40细胞中表达与NLS突变体融合的绿色荧光蛋白（GFP），我们显示了NLS对细胞增殖的重要作用。此外，我们表明，这两个集群的碱性氨基酸和一个接头的二分NLS是必不可少的，足以为核输入的GFP。外源性cmatr 3拯救了HeLa细胞，其中人matrin 3被RNA干扰抑制，但在碱性氨基酸簇或接头处含有缺失的cmatr 3不能。(c)2006年爱思唯尔公司All rights reserved.
Matrin 3, a nuclear matrix protein has potential (1) to withhold promiscuously edited RNAs within the nucleus in cooperation with p54(nrb) and PSF, (2) to mediate NMDA-induced neuronal death, and (3) to modulate promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). We identified a bipartite nuclear localization signal (NLS) of chicken matrin 3 (cmatr3) at residues 583-602. By expressing green fluorescent protein (GFP) fused to the NLS mutant in chicken DT40 cells, we showed an essential role of the NLS for cell proliferation. Furthermore, we showed that both clusters of basic amino acids and a linker of the bipartite NLS were essential and sufficient for the nuclear import of GFP. Exogenous cmatr3 rescued the HeLa cells where human matrin 3 was suppressed by RNA interference, but cmatr3 containing deletions at either of the basic amino acid clusters or the linker could not. (c) 2006 Elsevier Inc. All rights reserved.