CONCENTRATIONS OF GLYCERIDES AND PHOSPHOLIPIDS IN RAT HEART AND GASTROCNEMIUS MUSCLES - EFFECTS OF ALLOXAN-DIABETES AND PERFUSION
CONCENTRATIONS OF GLYCERIDES AND PHOSPHOLIPIDS IN RAT HEART AND GASTROCNEMIUS MUSCLES - EFFECTS OF ALLOXAN-DIABETES AND PERFUSION
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DOI：
10.1042/bj1040416
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发表时间：
1967-01-01
影响因子：
4.1
通讯作者：
RANDLE, PJ
中科院分区：
文献类型：
--
作者：
DENTON, RM;RANDLE, PJ
1.本文介绍了大鼠心脏和腓肠肌中脂类的提取方法，以及甘油单酯、甘油二酯、甘油三酯和磷脂磷的测定方法。2.在正常动物的心脏中，发现的浓度为：甘油单酯，0.6;甘油二酯，0.1;甘油三酯，12.6 μ mol甘油甘油酯/g。干肌肉;磷脂，171微克。磷脂磷原子/克。干燥的肌肉。腓肠肌中甘油酯的浓度与心肌相似，但磷脂的浓度较低（64μg磷脂磷原子/g）。干肌肉）。3.四氧嘧啶糖尿病增加了肌肉中的甘油三酯浓度的两倍。这种增加被证明是依赖于心脏的生长激素和皮质醇的可用性，但不对膳食脂质的可用性。心脏中的总甘油酯在48和72小时后增加。饥饿，但96小时后没有。在饥饿和糖尿病中观察到的甘油酯浓度的变化与磷脂浓度的显著变化无关。这表明，游离脂肪酸在糖尿病中的动员导致额外的甘油酯在肌肉中的合成。4.灌注期间心脏中的甘油酯脂肪酸对呼吸的可能贡献已根据灌注期间甘油酯的净损失以及脂解和酯化的相对速率计算，并与氧消耗平衡所需的脂肪酸氧化（葡萄糖或糖原葡萄糖氧化中未利用的氧）进行比较。在正常或糖尿病心脏灌注葡萄糖和胰岛素的甘油分解可以解释氧消耗的平衡。在无底物灌注的正常心脏中，氧消耗的平衡并不完全由甘油酯的分解来解释。
1. Methods are described for the extraction of lipid and assay of mono-, di- and tri-glyceride glycerol and phospholipid phosphorus in rat heart and gastrocnemius muscles. 2. In hearts from normal animals, concentrations found were: monoglyceride, 0·6; diglyceride, 0·1; triglyceride, 12·6μmoles of glyceride glycerol/g. of dry muscle; phospholipid, 171μg.atoms of phospholipid phosphorus/g. of dry muscle. Concentrations of glycerides in gastrocnemius muscle were similar to heart muscle but those of phospholipids were lower (64μg.atoms of phospholipid phosphorus/g. of dry muscle). 3. Alloxan-diabetes increased the concentration of triglyceride in the muscles twofold. This increase was shown to be dependent in the heart on the availability of growth hormone and cortisol but not on the availability of dietary lipid. Total glyceride in the heart was increased after 48 and 72hr. starvation but not after 96hr. Changes in glyceride concentration seen in starvation and diabetes were not associated with significant changes in phospholipid concentration. It is suggested that mobilization of free fatty acids in diabetes leads to the synthesis of additional glyceride in muscle. 4. The possible contribution of glyceride fatty acid in the heart to respiration during perfusion has been calculated from the net loss of glyceride during perfusion, and also from the relative rates of lipolysis and esterification and compared with oxidation of fatty acid required for the balance of oxygen consumption (oxygen not utilized in the oxidation of glucose or glycogen glucose). In the normal or diabetic heart perfused with glucose and insulin the breakdown of glyceride can account for the balance of oxygen consumption. In the normal heart perfused without substrate the balance of oxygen consumption is not entirely accounted for by the breakdown of glyceride.