Early treadmill exercise increases macrophage migration inhibitory factor expression after cerebral ischemia/reperfusion
Early treadmill exercise increases macrophage migration inhibitory factor expression after cerebral ischemia/reperfusion
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DOI：
10.4103/1673-5374.251330
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发表时间：
2019-07-01
影响因子：
6.1
通讯作者：
Kim, Dae Yul
中科院分区：
文献类型：
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作者：
Chang, Min Cheol;Park, Chae Ri;Kim, Dae Yul
巨噬细胞移动抑制因子（MIF）在缺血性脑卒中中的神经保护作用很少被评价。本研究旨在探讨早期平板运动对缺血性脑卒中恢复的影响，并确定这些影响是否与缺血区MIF和脑源性神经营养因子（BDNF）的表达水平有关。将40只雄性SD大鼠随机分为缺血运动组（MCAO-Ex，n = 10）、缺血安静组（MCAO-St，n = 10）、假手术运动组（Sham-Ex，n = 10）和假手术安静组（Sham-St，n = 10）。MCAO-Ex和MCAO-St组进行MCAO 60分钟，而Sham-Ex和Sham-St组进行相同的手术，没有MCAO。然后，MCAO-Ex和Sham-Ex组的大鼠在跑步机上跑步30分钟，每天一次，连续5天。再灌注后，与MCAO-St组相比，MCAO-Ex组梗死周围区域的悬吊时间较长，MRI测定的相对各向异性分数较高。MCAO-Ex组梗死周围区MIF和BDNF表达水平上调。增加的MIF和BDNF水平与梗死周围区域的相对分数各向异性变化呈正相关。Sham-Ex组和Sham-St组梗死周围区的MIF和BDNF水平无显著差异。我们的研究表明，早期运动（MCAO后48小时开始）可以改善缺血性中风后运动和神经元的恢复。脑梗死灶周围半暗区MIF和BDNF水平的升高可能是脑梗死后神经功能恢复增强的机制之一。所有实验均由韩国牙山医学中心的机构动物护理和使用委员会批准（2016-12-126）。
The neuroprotective function of macrophage migration inhibitory factor (MIF) in ischemic stroke was rarely evaluated. This study aimed to investigate the effects of early treadmill exercise on recovery from ischemic stroke and to determine whether these effects are associated with the expression levels of MIF and brain-derived neurotrophic factor (BDNF) in the ischemic area. A total of 40 male Sprague-Dawley rats were randomly assigned to the ischemia and exercise group [middle cerebral artery occlusion (MCAO)-Ex, n = 10), ischemia and sedentary group (MCAO-St, n = 10), sham-surgery and exercise group (Sham-Ex, n = 10), or sham-surgery and sedentary group (Sham-St, n = 10). The MCAO-Ex and MCAO-St groups were subjected to MCAO for 60 minutes, whereas the Sham-Ex and Sham-St groups were subjected to an identical operation without MCAO. Rats in the MCAO-Ex and Sham-Ex groups then ran on a treadmill for 30 minutes once a day for 5 consecutive days. After reperfusion, the hanging time tested by the wire hang test was longer and the relative fractional anisotropy determined by MRI was higher in the peri-infarct region of the MCAO-Ex group compared with the MCAO-St group. The expression levels of MIF and BDNF in the peri-infarct region were upregulated in the MCAO-Ex group. Increased MIF and BDNF levels were positively correlated with relative fractional anisotropy changes in the peri-infarct region. There was no significant difference in the levels of MIF and BDNF in the peri-infarct region between the Sham-Ex and Sham-St groups. Our study demonstrated that early exercise (initiated 48 hours after the MCAO) could improve motor and neuronal recovery after ischemic stroke. Furthermore, the increased levels of MIF and BDNF in the peri-infarct region (penumbra) may be one of the mechanisms of enhanced neurological function recovery. All experiments were approved by the Institutional Animal Care and Use Committee in Asan Medical Center in South Korea (2016-12-126).