Use of complexing reagents as additives to the eluent for optimization of separation selectivity in high-performance chelation ion chromatography.
Use of complexing reagents as additives to the eluent for optimization of separation selectivity in high-performance chelation ion chromatography.
复制标题
使用络合试剂作为洗脱液的添加剂来优化高性能螯合离子色谱中的分离选择性。
DOI：
10.1016/j.chroma.2008.10.010
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发表时间：
2008
影响因子：
4.1
通讯作者：
P. Nesterenko
中科院分区：
文献类型：
--
作者：
Phil Jones;P. Nesterenko
本文详细研究了高效络合离子色谱柱分离多种金属离子与多种络合淋洗液的选择性特性。这表明，利用洗脱液和表面键合基团中配体之间的竞争金属络合作用，可以广泛地控制特定应用中金属离子基团的保留顺序和选择性系数。首先给出了在亚氨基二乙酸(IDA)硅胶结合底物上发现的简单非络合淋洗液的金属分离特性的指示，然后说明了使用络合淋洗剂可以实现的选择性变化。使用一种新的方法，所选择的洗脱剂配体的金属络合物与表面键合的IDA金属络合物的对数β1图被发现是有用的指示剂，在分离过程中金属可能表现出异常的选择性变化。给出了三种选定的络合洗脱剂，即草酸、吡啶甲酸和氯化物，单独或混合使用时的金属分离实例色谱图。值得一提的是，铜(II)与吡啶甲酸、Fe(III)与Fe(II)与草酸、铅与二吡啶甲酸、Cd与氯化物的保留控制效果非常好。
This paper details a study of the selectivity characteristics of high-performance chelation ion chromatography when separating a range of metal ions with a number of complexing eluents. It shows that exploitation of competitive metal complexation between ligands in the eluent and surface bonded chelating groups allows a wide range of control over the retention order and selectivity coefficients of groups of metal ions for specific applications. An indication of the metal separation characteristics found for simple non-complexing eluents on iminodiacetic acid (IDA) silica bonded substrates is given first, followed by an illustration of the selectivity changes that can be achieved by using complexing eluents. Using a novel approach, plots of logβ1of the metal complexes of a chosen eluent ligand against the surface bonded IDA metal complexes were found to be useful indicators of which metals may show unusual selectivity changes during separation. Example chromatograms of metal separations are given for three selected complexing eluent reagents, namely, oxalic acid, picolinic acid, and chloride, either singly or in admixture. For special mention it was found that very specific retention control could be achieved for Cu(II) with picolinic acid, Fe(III) and Fe(II) speciation with oxalic acid, Pb with dipicolinic acid and Cd with chloride.