A Tryptophan Prenyltransferase with Broad Substrate Tolerance from Bacillus subtilis subsp. natto
A Tryptophan Prenyltransferase with Broad Substrate Tolerance from Bacillus subtilis subsp. natto
复制标题
来自枯草芽孢杆菌亚种的具有广泛底物耐受性的色氨酸异戊二烯转移酶。
DOI：
10.1002/cbic.201800174
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发表时间：
2018
期刊：
影响因子：
3.2
通讯作者：
Tian Tian
中科院分区：
文献类型：
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作者：
Abe Ikuro;Sugita Tomotoshi;Okada Masahiro;Nakashima Yu;Tian Tian
枯草芽孢杆菌纳豆亚种分泌 ComX 纳豆信息素作为群体感应信息素，产生用于生物膜形成的聚-γ-谷氨酸。信息素的氨基酸序列为Lys-Trp-Pro-Pro-Ile-Glu，色氨酸残基用法尼基进行翻译后修饰，形成三环支架。与其他芽孢杆菌 ComX 信息素不同，色氨酸残基远离前体肽 ComXnatto 的 C 末端。在这里，我们报告了 ComQnatto 的功能分析，它催化独特的法呢基转移反应。 ComQnatto 不仅可以识别全长 ComXnatto，还可以识别 N 端和/或 C 端截短的 ComXnatto 类似物，甚至可以在体外用法尼基修饰单个色氨酸。这些结果与计算的动力学参数一起表明，ComQnattodoes 不需要前导序列来识别底物，并且是一种有前途的酶，具有广泛的底物耐受性，可用于合成各种异戊二烯化色氨酸衍生物。
Bacillus subtilissubsp.nattosecretes the ComXnattopheromone as a quorum‐sensing pheromone to produce poly‐γ‐glutamate for biofilm formation. The amino‐acid sequence of the pheromone is Lys‐Trp‐Pro‐Pro‐Ile‐Glu, and the tryptophan residue is post‐translationally modified with a farnesyl group to form a tricyclic scaffold. Unlike otherBacillusComX pheromones, the tryptophan residue is distant from the C‐terminal end of the precursor peptide ComXnatto. Here, we report the functional analysis of ComQnatto, which catalyzes a unique farnesyl‐transfer reaction. ComQnattorecognizes not only full‐length ComXnattobut also N‐ and/or C‐terminal truncated ComXnattoanalogues and even a single tryptophan for modification with a farnesyl group in vitro. These results, together with the calculated kinetic parameters, suggest that ComQnattodoes not require a leader sequence for substrate recognition and is a promising enzyme with broad substrate tolerance for the synthesis of various prenylated tryptophan derivatives.