I5M:: 3D widefield light microscopy with better than 100 nm axial resolution
I5M:: 3D widefield light microscopy with better than 100 nm axial resolution
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DOI：
10.1046/j.1365-2818.1999.00576.x
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发表时间：
1999-07-01
影响因子：
2
通讯作者：
Sedat, JW
中科院分区：
文献类型：
--
作者：
Gustafsson, MGL;Agard, DA;Sedat, JW
三维宽场荧光显微镜的轴向分辨率提高了七倍，采用了一种新颖的干涉技术，其中使用两个相对的物镜同时从两侧观察和/或照明样品。激发光和发射光中的独立干涉效应可以获取比传统宽场或共焦显微镜更高分辨率的样品轴向信息。在这里，我们报告了复杂生物样品上这种分辨率性能的实验验证。
Sevenfold improved axial resolution has been achieved in three-dimensional widefield fluorescence microscopy, using a novel interferometric technique in which the sample is observed and/or illuminated from both sides simultaneously using two opposing objective lenses. Separate interference effects in the excitation light and the emitted light give access to higher resolution axial information about the sample than can be reached by conventional widefield or confocal microscopes. Here we report the experimental verification of this resolution performance on complex biological samples.