Identification of MdDof genes in apple and analysis of their response to biotic or abiotic stress
Identification of MdDof genes in apple and analysis of their response to biotic or abiotic stress
复制标题
苹果MdDof基因的鉴定及其对生物或非生物胁迫的响应分析
DOI：
10.1071/fp17288
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发表时间：
2018-01-01
影响因子：
3
通讯作者：
Li, Tianzhong
中科院分区：
文献类型：
--
作者：
Yang, Qing;Chen, Qiuju;Li, Tianzhong
Dof结构域蛋白作为一个典型的植物特异性转录因子家族，参与了从单细胞衣原体到高等植物的多种生物学过程。然而，有有限的报告MdDof（苹果Borkh。DNA结合一个锌指）结构域蛋白在果树，特别是在苹果。在这项研究中，我们确定了苹果基因组中的54个推定的Dof转录因子。我们分析了每个MdDof基因的基因结构、蛋白质基序和染色体位置。接下来，我们描述了所有54个MdDofs在不同的非生物和生物胁迫条件下的表达模式。MdDof 6，26不仅在生物/非生物胁迫中起重要作用，而且可能参与多种分子功能。进一步研究发现，在花发育和花粉管生长过程中，MdDof 24的相对表达量迅速增加，基因本体分析表明MdDof 24参与了化学反应和花发育途径。这些结果为苹果MdDof基因的功能研究提供了有用的线索，也为其他植物Dof锌指基因的研究提供了参考。
As a classic plant-specific transcription factor family - the Dof domain proteins - are involved in a variety of biological processes in organisms ranging from unicellular Chlamydomonas to higher plants. However, there are limited reports of MdDof (Malus domestica Borkh. DNA-binding One Zinc Finger) domain proteins in fruit trees, especially in apple. In this study we identified 54 putative Dof transcription factors in the apple genome. We analysed the gene structures, protein motifs, and chromosome locations of each of the MdDof genes. Next, we characterised all 54 MdDofs their expression patterns under different abiotic and biotic stress conditions. It was found that MdDof6,26 not only played an important role in the biotic/abiotic stress but may also be involved in many molecular functions. Further, both in flower development and pollen tube growth it was found that the relative expression of MdDof24 increased rapidly, also with gene ontology analysis it was indicated that MdDof24 was involved in the chemical reaction and flower development pathways. Taken together, our results provide useful clues as to the function of MdDof genes in apple and serve as a reference for studies of Dof zinc finger genes in other plants.