CHARACTERIZATION AND DISTRIBUTION OF INTERSTITIAL CELL-TYPES IN THE RENAL-CORTEX OF RATS
CHARACTERIZATION AND DISTRIBUTION OF INTERSTITIAL CELL-TYPES IN THE RENAL-CORTEX OF RATS
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DOI：
10.1038/ki.1994.95
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发表时间：
1994-03-01
影响因子：
19.6
通讯作者：
LEHIR, M
中科院分区：
文献类型：
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作者：
KAISSLING, B;LEHIR, M
尽管皮质管周间质的细胞成分具有生理和病理生理学的重要性，但其细胞成分的精确分类仍然缺失。通过结合电子显微镜和免疫组织化学，我们试图更好地识别和定位大鼠肾脏中不同的间质细胞类型，特别关注皮质。将针对常见白细胞抗原 (OX1)、巨噬细胞抗原 (ED 2)、MHC II 类抗原 (OX6) 和 ecto-5'-核苷酸酶的抗体应用于低温切片，后两种抗体另外用于电子显微镜检查。鉴定出四组细胞类型，它们表现出不同的抗原模式、分布和形态特征。它们被鉴定为成纤维细胞、树突状细胞、巨噬细胞和淋巴细胞。成纤维细胞和树突状细胞构成了皮质和外髓质中最丰富的细胞类型，它们相当均匀地分布在整个管周间质的这些区域，而巨噬细胞非常稀少。后者在大血管和盆壁的结缔组织中含量丰富。淋巴细胞的发生率一般较低。这些数据一方面可能有助于肾间质细胞的进一步表型表征，另一方面有助于评估这些细胞在病理过程中的作用。
A precise classification of the cellular components of the cortical peritubular interstitium is missing in spite of its physiological and pathophysiological importance. By combining electronmicroscopy and immunohistochemistry we attempted to better identify and localize the different interstitial cell types in rat kidney, with special attention to the cortex. Antibodies against common leukocyte antigen (OX1), a macrophage antigen (ED 2), MHC class II antigen (OX6) and ecto-5'-nucleotidase were applied to cryostat sections, The two latter antibodies were used additionally in electron microscopy. Four groups of cell types were identified that displayed distinct antigenic patterns, distributions and morphological features. They were identified as fibroblasts, dendritic cells, macrophages and lymphocytes. Fibroblasts and dendritic cells constituted the most abundant cell types in the cortex and outer medulla, and they were rather uniformly distributed in these zones throughout the peritubular interstitium, where macrophages were very scarce. The latter were abundant in the connective tissue of large vessels and of the pelvic wall. The incidence of lymphocytes was generally low. These data may be helpful on the one hand for the further phenotypic characterization of renal interstitial cells and on the other hand for the evaluation of the roles of those cells in pathological processes.