Regulation of catecholamine synthesis in the rat brain in vitro by cyclic AMP
Regulation of catecholamine synthesis in the rat brain in vitro by cyclic AMP
复制标题
环AMP对大鼠脑体外儿茶酚胺合成的调节
作者：
Jane E. Harris;V. Morgenroth;Robert H. Roth;Ross J. Baldessarini
在松果体1、上级颈神经节2以及脑切片3、4和匀浆5中，儿茶酚胺刺激腺苷环-3 ′，5 ′-单磷酸（cAMP）的产生的证据表明，cAMP在外周和中枢肾上腺素能神经传递中具有重要的突触后功能。在腹下神经-输精管连接6和肾上腺髓质7中也有证据表明，双丁酰环AMP增加去甲肾上腺素的释放，表明环AMP在调节肾上腺素能末梢中的儿茶酚胺代谢中具有突触前作用。在中枢神经系统中，腺苷酸环化酶和磷酸二酯酶在相当大的程度上与孤立的神经末梢（突触体）部分8相关，其中假定的神经递质主要位于该部分。因此，它是感兴趣的，以确定是否环AMP也可能刺激儿茶酚胺营业额在大脑中，特别是在突触体制备的脑匀浆。
EVIDENCE that catecholamines stimulate the production of adenosine cyclic-3′,5′-monophosphate (cyclic AMP) in the pineal gland1, superior cervical ganglia2 and both in brain slices3,4 and homogenates5 has suggested that cyclic AMP has an important postsynaptic function in both peripheral and central adrenergic neurotransmission. Evidence has also been presented in the hypogastric nerve–vas deferens preparation6 and adrenal medulla7 that dibutyryl cyclic AMP increases the release of noradrenaline, suggesting a presynaptic role for cyclic AMP in modulating catecholamine metabolism in adrenergic terminals. In the central nervous system, adenylate cyclase and phosphodiesterase are, to a considerable extent, associated with the isolated nerve-ending (synaptosomal) fraction8 in which putative neurotransmitters are primarily localised. Thus it was of interest to determine whether cyclic AMP might also stimulate catecholamine turnover in the brain, especially in synaptosomes prepared from brain homogenates.