RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity-alkalinity stress.
RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity-alkalinity stress.
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DOI：
10.1016/j.plaphy.2021.04.017
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发表时间：
2021-05
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影响因子：
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通讯作者：
Jiwen Xu;Z. Kang;Keyu Zhu;Dingkang Zhao;Yajing Yuan;Shichun Yang;Wentian Zhen;Xiaohui Hu
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文献类型：
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作者：
Jiwen Xu;Z. Kang;Keyu Zhu;Dingkang Zhao;Yajing Yuan;Shichun Yang;Wentian Zhen;Xiaohui Hu
盐碱胁迫是世界范围内番茄生产的一个限制因素。植物通过激活信号传导途径来感知盐碱胁迫以增加植物耐受性（Xu et al.，2020年）。本文研究了精胺（Spm）是否诱导番茄呼吸爆发氧化酶同源物1（RBOH 1）和过氧化氢（H2 O2）信号对盐碱胁迫的响应。结果表明，在正常条件下，外源Spm诱导了RBOH 1的表达和H2 O2的积累。因此，我们检测了H2 O2信号在番茄幼苗中的作用，发现外源H2 O2增加了盐碱胁迫下番茄幼苗中Cu/Zn-SOD、CAT 1、cAPX和GR 1的表达水平以及SOD（EC 1.15.1.1）、CAT（EC 1.11.1.6）、APX和GR（EC 1.6.4.2）的活性。1.11.1.11在盐碱胁迫下，DMTU处理的番茄幼苗叶片中丙二醛（MDA）含量、相对电导率和相对含水量增加，叶片黄化加速。在盐碱胁迫下，RBOH 1基因沉默降低了番茄幼苗Cu/Zn-SOD、CAT 1、cAPX和GR 1的表达水平，降低了SOD、CAT、APX和GR的活性。在盐碱胁迫下，外源Spm不能提高RBOH 1沉默番茄幼苗的RWC，也不能降低MDA含量。
Salinity–alkalinity stress is a limiting factor in tomato production in the world. Plants perceive salinity–alkalinity stress by activating signaling pathways to increase plant tolerance (Xu et al., 2020). Here, we investigated whether spermine (Spm) inducesrespiratory burstoxidasehomolog 1 (RBOH1)and hydrogen peroxide (H2O2) signaling in response to salinity–alkalinity stress in tomato. The results showed that exogenous Spm induced the expression ofRBOH1and the accumulation of H2O2under normal condition. Accordingly, we tested the function of H2O2signal in tomato seedlings and found that exogenous H2O2increased the expression levels ofCu/Zn-superoxide dismutase(Cu/Zn-SOD), catalase 1 (CAT1), cytosolic ascorbate peroxidase(cAPX), and glutathione reductase 1 (GR1)and the activities of SOD (EC 1.15.1.1), CAT (EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and GR (EC 1.6.4.2) in tomato seedlings under salinity–alkalinity stress. DMTU increased the malondialdehyde (MDA) content and relative electrical conductivity, and the relative water content (RWC), and accelerated leaf yellowing in tomato seedlings under salinity–alkalinity stress, even though we sprayed Spm on tomato leaves. We also found thatRBOH1silencing decreased the expression levels ofCu/Zn-SOD,CAT1,cAPX, andGR1and the activities of SOD, CAT, APX, and GR when tomato seedlings were under salinity–alkalinity stress. Exogenous Spm did not increase RWC and decrease MDA content inRBOH1silencing tomato seedlings under salinity–alkalinity stress.