Enhancement of hypothermic heart preservation with fructose 1,6-diphosphate
Enhancement of hypothermic heart preservation with fructose 1,6-diphosphate
复制标题
DOI：
10.1006/jsre.1999.5658
复制
发表时间：
1999-07-01
影响因子：
2.2
通讯作者：
Chien, S
中科院分区：
文献类型：
--
作者：
Niu, WY;Zhang, FT;Chien, S
背景。我们假设在低温心脏保存溶液中添加果糖 1,6-二磷酸 (FDP) 可以改善代谢恢复，因为它具有多种有益作用。材料和方法。二十只成年斯普拉格-道利大鼠被用来研究低温心脏保存。在全身麻醉下取出心脏并在4℃下在Euro-Collins溶液(30ml/kg)中保存8小时。在研究组 (N = 10) 中，将 FDP (5 mM) 添加到 Euro-Collins 溶液中。在对照组（N = 10）中，不添加 FDP。使用工作心脏模型在保存后研究心脏功能。检查了不同浓度的果糖 1,g-磷酸被动扩散通过卵磷脂酰胆碱多层囊泡 (MLV) 双层膜的能力。结果。研究组的心输出量范围为 17.0 +/- 1.9 至 24.9 +/- 1.6 ml/min，对照组为 2.0 +/- 1.0-12.3 +/- 1.7 ml/min，研究组的平均主动脉流量为 10.8 +/- 1.4 ml/min，对照组为 -1.3 +/- 1.6 ml/min，最大 LV 生成功率为 22.8 +/- 1.7 J/min 对比对照组为 10.1 +/- 1.6 J/min。研究组的冠状动脉流量、左心室每搏输出量、每搏功以及心肌耗氧量均远高于对照组。研究组的冠状血管阻力低于对照组。电子显微镜研究表明，对照组的许多肌细胞显示出肿胀的线粒体斑块，但在研究组中很少观察到。添加 50 mM FDP 导致 MLV 渗透性发生显着变化。囊泡外没有剂量的蔗糖缓冲液会导致MLV通透性发生显着变化。结论我们的结果表明，在Euro-Collins溶液中添加FDP可显着改善低温大鼠心脏的保存，并且FDP似乎可以穿过膜双层。 (C) 1999 年学术出版社。
Background. We hypothesized that the addition of fructose 1,6-diphosphate (FDP) to a hypothermic heart preservation solution could improve metabolic recovery because it has several beneficial effects.Materials and Methods. Twenty adult Sprague-Dawley rats were used to study hypothermic heart preservation. The hearts were removed under general anesthesia and preserved at 4 degrees C in Euro-collins solution (30 ml/kg) for 8 h. In the study group (N = 10), FDP (5 mM) was added to the Euro-Collins solution. In the control group (N = 10), no FDP was added. Heart function was studied after preservation using a working heart model. The ability of various concentrations of fructose 1,g-phosphate to passively diffuse through an egg phosphatidylcholine multilamellar vesicle (MLV) membrane bilayer was examined.Results. Cardiac output ranged from 17.0 +/- 1.9 to 24.9 +/- 1.6 ml/min in the study group vs 2.0 +/- 1.0-12.3 +/- 1.7 ml/min for controls, average aortic how was 10.8 +/- 1.4 ml/min in the study group vs -1.3 +/- 1.6 ml/min for controls, and maximum LV generated power was 22.8 +/- 1.7 J/min vs 10.1 +/- 1.6 J/min for controls. Coronary how, left ventricular stroke volume and stroke work, and myocardial oxygen consumption were much higher in the study group than in the control group. Coronary vascular resistance was lower in the study group than in the control group. Electron microscopic study indicated that many myocytes displayed patches of swollen mitochondria in the control group, but was rarely observed in the study group. The addition of 50 mM FDP caused substantial changes in MLV permeability. No dose of sucrose buffers outside the vesicles resulted in a significant changes of MLV permeability.Conclusions Our results indicate that the addition of FDP to Euro-Collins solution significantly improves hypothermic rat heart preservation, and FDP appeared to cross the membrane bilayer. (C) 1999 Academic Press.