Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis
Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis
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DOI：
10.1007/s12094-015-1364-1
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发表时间：
2016-03-01
影响因子：
3.4
通讯作者：
Duan, Ranhui
中科院分区：
文献类型：
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作者：
Xu, Feng;He, Hua;Duan, Ranhui
MicroRNA-200家族(miR-200F)一直被认为是失控的，参与多种肿瘤的转移过程。本研究检测了miR-200F在乳腺癌组织中的表达，并探讨其与临床病理特征，尤其是与淋巴结转移的关系。采用实时荧光定量聚合酶链式反应检测99对乳腺癌组织及癌旁正常组织中miR-200a、miR-200b、miR-200C、miR-141和miR-429的表达水平。通过Mann-Whitney检验、方差分析和操作特征(ROC)分析，发现miR-200F在癌组织中表达下调，miR-200a、miR-200b和miR-200c表达显著降低(p<；0.05)，而miR-141和miR-429在癌组织中的表达差异无统计学意义。此外，5组患者的miR-200F表达均随淋巴结转移率的增加而显著降低(p<；0.05)，当按淋巴结转移数(0；1~3；百万分之4)分为三组时，miR-200F的表达随淋巴结转移数的增加而逐渐降低；ROC显示miR-200b可以区分有淋巴结转移的患者和无淋巴结转移的患者，这些观察表明miR-200F在人BC中的下调与侵袭性表型有关，miR-200b可能有助于估计存在病理阳性淋巴结的可能性。
MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann-Whitney test, ANOVA, and operating characteristic (ROC) analysis.All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (p < 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (p < 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1-3; a parts per thousand yen4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.