Rational design and one-step formation of multifunctional gel transducer for simple fabrication of integrated electrochemical biosensors.
Rational design and one-step formation of multifunctional gel transducer for simple fabrication of integrated electrochemical biosensors.
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DOI：
10.1021/ac200942a
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发表时间：
2011-06
影响因子：
7.4
通讯作者：
Ping Yu;Heng Zhou;Hanjun Cheng;Qin Qian;L. Mao
中科院分区：
文献类型：
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作者：
Ping Yu;Heng Zhou;Hanjun Cheng;Qin Qian;L. Mao
本研究展示了一种新的策略，以简化生物传感器的制作，从而最大限度地减少生物传感器之间的偏差，通过合理的设计和一步形成的多功能凝胶电子换能器集成的所有元素，有效地转导的生物识别事件的信号读出，通过使用葡萄糖脱氢酶（GDH）为基础的电化学生物传感器为例。为了满足制备集成生物传感器和保持电子和离子电导率用于电子转导过程的要求，具有酶辅因子（即，氧化形式的烟酰胺腺嘌呤二核苷酸）作为阴离子，并用于与单壁碳纳米管形成巴基凝胶，其中亚甲基绿色电催化剂被稳定地封装用于烟酰胺腺嘌呤二核苷酸的氧化。以这种设计合理、一步成型的多功能凝胶为电传感器，通过将电极抛光到凝胶上，然后固定酶，简单地制备了基于GDH的电化学生物传感器。这种能力极大地简化了生物传感器的制造，提高了生物传感器的稳定性，并且更显著地，使生物传感器与生物传感器之间的偏差最小化。用同一电极重复测定葡萄糖和用同一方法制备的不同电极同时测定相同浓度葡萄糖的相对标准偏差分别为3.30%（n = 7）和4.70%（n = 6）。多功能凝胶基生物传感器的这些优异性能使它们能够很好地满足快速测量的迫切需要，例如环境监测、食品分析和临床诊断。
This study demonstrates a new strategy to simplify the biosensor fabrication and thus minimize the biosensor-to-biosensor deviation through rational design and one-step formation of a multifunctional gel electronic transducer integrating all elements necessitated for efficiently transducing the biorecognition events to signal readout, by using glucose dehydrogenase (GDH) based electrochemical biosensor as an example. To meet the requirements for preparing integrated biosensors and retaining electronic and ionic conductivities for electronically transducing process, ionic liquids (ILs) with enzyme cofactor (i.e., oxidized form of nicotinamide adenine dinucleotide) as the anion were synthesized and used to form a bucky gel with single-walled carbon nanotubes, in which methylene green electrocatalyst was stably encapsulated for the oxidation of nicotinamide adenine dinucleotide. With such kind of rationally designed and one-step-formed multifunctional gel as the electronic transducer, the GDH-based electrochemical biosensors were simply fabricated by polishing the electrodes onto the gel followed by enzyme immobilization. This capability greatly simplifies the biosensor fabrication, prolongs the stability of the biosensors, and, more remarkably, minimizes the biosensor-to-biosensor deviation. The relative standard deviations obtained both with one electrode for the repeated measurements of glucose and with the different electrodes prepared with the same method for the concurrent measurements of glucose with the same concentration were 3.30% (n = 7) and 4.70% (n = 6), respectively. These excellent properties of the multifunctional gel-based biosensors substantially enable them to well-satisfy the pressing need of rapid measurements, for example, environmental monitoring, food analysis, and clinical diagnoses.