Principles of cell-free genetic circuit assembly
Principles of cell-free genetic circuit assembly
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DOI：
10.1073/pnas.2135496100
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发表时间：
2003-10-28
影响因子：
11.1
通讯作者：
Libchaber, A
中科院分区：
文献类型：
--
作者：
Noireaux, V;Bar-Ziv, R;Libchaber, A
在转录翻译提取物中构建了无细胞遗传回路元件。我们设计了转录激活和抑制级联，其中每个阶段的蛋白质产物是驱动或阻止以下阶段所需的输入。尽管我们可以找到单个阶段的线性响应区域，但级联到后续阶段需要在非线性方案中工作。由于翻译机械上的瓶颈，每个阶段都会发生大幅度的时间延迟和产量的急剧下降。 RNA消息的更快营业额可以减轻基因之间的竞争，并根据输入和参数的变化稳定输出。
Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in nonlinear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage because of a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters.