Bioorthogonal chemistry: strategies and recent developments.
Bioorthogonal chemistry: strategies and recent developments.
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DOI：
10.1039/c3cc44272a
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发表时间：
2013-12-07
期刊：
影响因子：
--
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Ramil CP;Lin Q
利用共价化学来追踪天然环境中的生物分子是生物正交化学的一个研究热点，近年来引起了化学生物学家和有机化学家的极大兴趣。为了促进生物正交化学在生物医学研究中的广泛应用，过去几年的主要工作集中在优化一些已知的生物正交反应，特别是在反应动力学改进、新的遗传编码系统和生物成像的荧光反应方面。在这些优化过程中，出现了三种策略，包括在环加成反应中使用环应变来激活底物，发现新的配体和用于加速金属催化反应的特殊底物，以及设计具有预荧光团结构的底物，以便在选择性生物正交反应后快速“开启”荧光。此外，还报道了基于修饰或完全前所未有的反应物对的新的生物正交反应。最后，人们越来越关注互斥生物正交反应的发展及其在细胞培养中对生物分子进行多重标记的应用。在这篇专题文章中，我们希望通过选择突出上述策略的例子来介绍生物正交反应的最新进展。考虑到生物正交化学的发展越来越复杂，我们努力预测几个令人兴奋的机会，生物正交化学可以在不久的将来为生物学做出独特的贡献。
The use of covalent chemistry to track biomolecules in their native environment—a focus of bioorthogonal chemistry—has received considerable interests recently among chemical biologists and organic chemists alike. To facilitate wider adoption of bioorthogonal chemistry in biomedical research, a central effort in the last few years has been focused on the optimization of a few known bioorthogonal reactions, particularly with respective to reaction kinetics improvement, novel genetic encoding systems, and fluorogenic reactions for bioimaging. During these optimizations, three strategies have emerged, including the use of ring strain for substrate activation in the cycloaddition reactions, the discovery of new ligands and privileged substrates for accelerated metal-catalysed reactions, and the design of substrates with pre-fluorophore structures for rapid “turn-on” fluorescence after selective bioorthogonal reactions. In addition, new bioorthogonal reactions based on either modified or completely unprecedented reactant pairs have been reported. Finally, increasing attention has been directed toward the development of mutually exclusive bioorthogonal reactions and their applications in multiple labeling of a biomolecule in cell culture. In this feature article, we wish to present the recent progress in bioorthogonal reactions through the selected examples that highlight the above-mentioned strategies. Considering increasing sophistication in bioorthogonal chemistry development, we strive to project several exciting opportunities where bioorthogonal chemistry can make a unique contribution to biology in near future.
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影响因子：
15
作者：
Brustad EM;Lemke EA;Schultz PG;Deniz AA
通讯作者：
Deniz AA
影响因子：
15
作者：
Blackman, Melissa L.;Royzen, Maksim;Fox, Joseph M.
通讯作者：
Fox, Joseph M.
DOI：
10.1007/s10482-006-9088-4
发表时间：
2006-11-01
影响因子：
2.6
作者：
De Windt, Wim;Boon, Nico;Verstraete, Willy
通讯作者：
Verstraete, Willy
DOI：
10.1039/c3cc45752d
发表时间：
2013-11-04
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
An P;Yu Z;Lin Q
通讯作者：
Lin Q
影响因子：
16.6
作者：
Brustad, Eric;Bushey, Mark L.;Schultz, Peter G.
通讯作者：
Schultz, Peter G.