Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (Glossina morsitans morsitans).
Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (Glossina morsitans morsitans).
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DOI：
10.1186/1471-2164-11-160
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发表时间：
2010-03-09
期刊：
影响因子：
4.4
通讯作者：
Aksoy S
中科院分区：
文献类型：
--
作者：
Attardo GM;Ribeiro JM;Wu Y;Berriman M;Aksoy S
采采蝇是非洲锥虫的媒介，进行胎生繁殖（活后代的沉积）。这种繁殖策略导致大量的母体投资和在雌性生命周期内少量后代的沉积。舌蝇的生殖生物学已在生理水平上进行了研究;但舌蝇生殖的分子分析还需要更深入的研究。为建立采采蝇生殖分子生物学研究的基础，构建了采采蝇雌蝇生殖组织和子宫内发育阶段的cDNA文库。对3438个表达序列标签进行测序和分析。对1391个重叠群的非冗余目录进行分析，得到520个预测蛋白。其中475个蛋白质是全长蛋白质。我们预测，其中412代表细胞质蛋白，而57分泌。这些蛋白质与其他组织特异性采采蝇cDNA文库（唾液腺，脂肪体/乳腺，和中肠）的比较确定了51个是独特的生殖/未成熟的cDNA文库。11个独特的蛋白质是同源性的NR数据库中的未表征的推定蛋白质，这表明在其他昆虫中与生殖功能相关的新基因的鉴定（假设保守）。该分析还产生了七个推定的蛋白质，与公共数据库中存在的序列（未知基因）没有显著的同源性。这些蛋白质可能代表与采采蝇的胎生生殖周期相关的独特功能。对假设的保守和未知重叠群进行RT-PCR分析，以确定这些基因表达的基本组织和阶段特异性。本文确定了51个推定的蛋白质特异性采采蝇生殖/不成熟EST库。这些蛋白质中有11个对应于假设的保守基因，7个蛋白质是采采蝇特异性的。
Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (Glossina morsitans morsitans) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed. Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes. This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.