CXCR7: A novel tumor endothelial marker in renal cell carcinoma
CXCR7: A novel tumor endothelial marker in renal cell carcinoma
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DOI：
10.1111/j.1440-1827.2012.02792.x
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发表时间：
2012-05-01
影响因子：
2.2
通讯作者：
Hida, Kyoko
中科院分区：
文献类型：
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作者：
Maishi, Nako;Ohga, Noritaka;Hida, Kyoko
肿瘤血管生成是实体瘤发生、发展和转移的必要条件。肿瘤血管在形态上不同于它们的正常对应物。在这项研究中，我们分离肿瘤内皮细胞（TEC），并揭示其异常。我们通过微阵列分析比较了TEC和正常内皮细胞（NECs）的基因表达谱，发现有几个基因在TEC中上调。TECs中趋化因子受体CXCR7 mRNA的表达高于NECs。然而，关于CXCR7在肾细胞癌的肿瘤血管中的表达的信息有限。CXCR7及其配体CXCL12与肿瘤细胞存活有关。本研究探讨了CXCR7在肾细胞癌（RCC）血管中的表达。实时荧光定量PCR显示CXCR7在体外培养的TECs中的表达水平高于体外培养的NECs。此外，与小鼠TEC相似，免疫染色显示CXCR7在人肿瘤血管中体内强表达。这些发现表明CXCR 7是一种新型TEC标志物，也是RCC抗血管生成治疗的靶点。
Tumor angiogenesis is necessary for progression and metastasis of solid tumor. Tumor blood vessels are morphologically different from their normal counterparts. In this study, we isolated tumor endothelial cells (TECs) and revealed their abnormalities. We have compared the gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and found that several genes were upregulated in TECs. Expression of the chemokine receptor CXCR7 mRNA was higher in TECs than in NECs. However, information regarding the expression of CXCR7 in the tumor vessels of renal cell carcinoma is limited. CXCR7 and its ligand CXCL12 have been implicated in tumor cell survival. In this study, the expression of CXCR7 in the tumor vessels of renal cell carcinoma (RCC) was investigated. Real-time PCR revealed higher expression level of CXCR7 in cultured TECs than in cultured NECs. Furthermore, similar to mouse TECs, immunostaining revealed strong expression of CXCR7 in vivo in human tumor vessels. These findings suggest that CXCR7 is a novel TEC marker and a target for antiangiogenic therapy for RCC.