Additional Evidence That Transaldolase Exchange, Isotope Discrimination During the Triose-Isomerase Reaction, or Both Occur in Humans Effects of Type 2 Diabetes
Additional Evidence That Transaldolase Exchange, Isotope Discrimination During the Triose-Isomerase Reaction, or Both Occur in Humans Effects of Type 2 Diabetes
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DOI：
10.2337/db08-1300
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发表时间：
2009-07-01
期刊：
影响因子：
7.7
通讯作者：
Rizza, Robert A.
中科院分区：
文献类型：
--
作者：
Basu, Rita;Chandramouli, Visvanthan;Rizza, Robert A.
目的 - 确定血浆葡萄糖中碳 5 和碳 3 (C5/C3) 上的氘富集是否受到糖异生以外的过程的影响，如果是，这些过程是否会因 2 型糖尿病而改变。 研究设计和方法 - 在这项研究中，10 名肥胖糖尿病患者和 10 名肥胖非糖尿病受试者静脉注射富含 [3,5-H-2(2)] 半乳糖的药物使用对乙酰氨基酚葡萄糖醛酸法通过核磁共振测量，C5 与 C3 的比率为 1.0，并且氘在血浆葡萄糖的 C5 和 C3 上富集。结果-在所有糖尿病和非糖尿病受试者中，葡萄糖的 C5 和 C3 上的氘富集比率 < 1 (P < 0.001)，导致组间 C5 与 C3 比率的平均值 +/- SE 没有差异（分别为 0.81 +/- 0.01 与 0.79 +/- 0.01）。结论 - C5 与 C3 葡萄糖比率为
OBJECTIVE-To determine whether deuterium enrichment on carbons 5 and 3 (C5/C3) in plasma glucose is influenced by processes other than gluconeogenesis and, if so, whether these processes are altered by type 2 diabetes.RESEARCH DESIGN AND METHODS-In this study, 10 obese diabetic and 10 obese nondiabetic subjects were infused intravenously with [3,5-H-2(2)] galactose enriched at a C5-to-C3 ratio of 1.0 as well as the enrichment of deuterium on C5 and C3 of plasma glucose, measured with nuclear magnetic resonance using the acetaminophen glucuronide method.RESULTS-The ratio of deuterium enrichment on C5 and C3 of glucose was < 1 (P < 0.001) in all of the diabetic and nondiabetic subjects, resulting in a means +/- SE C5-to-C3 ratio that did not differ between groups (0.81 +/- 0.01 vs. 0.79 +/- 0.01, respectively).CONCLUSIONS-That the C5-to-C3 glucose ratio is