Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: Implications for developmental potential
Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: Implications for developmental potential
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DOI：
10.1016/j.ydbio.2006.07.022
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发表时间：
2006-11-01
影响因子：
2.7
通讯作者：
Schatten, Heide
中科院分区：
文献类型：
--
作者：
Katayama, Mika;Zhong, Zhisheng;Schatten, Heide
对孤雌生殖、受精和体细胞核移植(SCNT)后猪卵母细胞线粒体分布和微管结构进行了观察。我们的结果表明，线粒体通过微管从卵母细胞的皮质转移到核周区域，这些微管既构成了体外受精(IVF)卵母细胞的精子核心，也起源于SCNT卵母细胞的供体细胞中心体。SCNT卵母细胞将线粒体移位到核周的能力低于IVF卵母细胞。精子诱导的激活而不是电激活的SCNT卵母细胞以及卵母细胞纺锤体的存在增强了核周围线粒体与重构核的联系，而在精子穿透之前去除卵母梭则减少了线粒体与男性原核的联系，对微管没有明显的影响。我们认为，精子和卵母细胞纺锤体来源的因素可能影响猪卵母细胞体外受精和胚胎移植后受精卵微管移位线粒体的能力。线粒体与原核结合与IVF后胚胎发育呈正相关。SCNT卵母细胞中线粒体与细胞核结合的减少可能是克隆效率低的原因之一，这可以通过添加生理受精过程中通常存在的精子衍生因子来纠正。(C)2006 Elsevier Inc.保留所有权利。
Mitochondrial distribution and microtubule organization were examined in porcine oocytes after parthenogenesis, fertilization and somatic cell nuclear transfer (SCNT). Our results revealed that mitochondria are translocated from the oocyte's cortex to the perinuclear area by microtubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate from the donor cell centrosomes in SCNT oocytes. The ability to translocate mitochondria to the perinuclear area was lower in SCNT oocytes than in IVF oocytes. Sperm-induced activation rather than electrical activation of SCNT oocytes as well as the presence of the oocyte spindle enhanced perinuclear mitochondrial association with reconstructed nuclei, while removal of the oocyte spindle prior to sperm penetration decreased mitochondrial association with male pronuclei without having an apparent effect on microtubules. We conclude that factors derived from spermatozoa and oocyte spindles may affect the ability of zygotic microtubules to translocate mitochondria after IVF and SCNT in porcine oocytes. Mitochondrial association with pronuclei was positively related with embryo development after IVF. The reduced mitochondrial association with nuclei in SCNT oocytes may be one of the reasons for the low cloning efficiency which could be corrected by adding yet to be identified, sperm-derived factors that are normally present during physiological fertilization. (c) 2006 Elsevier Inc. All rights reserved.