Teaching protein purification and characterization techniques - A student-initiated, project-oriented biochemistry laboratory course
Teaching protein purification and characterization techniques - A student-initiated, project-oriented biochemistry laboratory course
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DOI：
10.1021/ed085p1250
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发表时间：
2008-09-01
影响因子：
3
通讯作者：
MacDonald, Gina
中科院分区：
文献类型：
--
作者：
MacDonald, Gina
本报告描述了一个完全以项目为导向的生物化学实验室。高年级的生物和化学专业的学生在团队中工作，以纯化他们选择的蛋白质。在学生小组完成文献检索，订购试剂和缓冲液后，他们将继续学习基本的蛋白质纯化和生化技术。随着蛋白质纯化的进展，学生们遇到了许多与试图重现已发表的蛋白质纯化相关的常见问题。学生获得宝贵的解决问题的技能，并在研究小组的环境中工作。课程的高潮需要蛋白质的一些表征，正式的实验室报告和最终报告。学生使用活性分析或红外或圆二色性等技术来表征蛋白质，以研究蛋白质结构或展开。日常实验室的经验类似于一个活跃的本科研究实验室。该实验室是非结构化的，致力于整合研究和教育，并通过将学生暴露在一个完全开放的，基于探究的实验室中来留住学生。
This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques as needed during the semester. As the protein purifications progress the students encounter many common problems associated with attempting to reproduce published protein purifications. The students gain valuable problem-solving skills and work in a research group environment. The culmination of the course requires some characterization of the protein, a formal laboratory report, and a final presentation. Students characterize their proteins using activity assays or techniques such as infrared or circular dichroism to study protein structure or unfolding. Day-to-day laboratory experiences resemble an active undergraduate research laboratory. This laboratory is unstructured and works to integrate research and education and retain students in the sciences by exposing them to a completely open-ended, inquiry-based laboratory.