Dietary restriction interferes with oxidative status and intrinsic intestinal innervation in aging rats
Dietary restriction interferes with oxidative status and intrinsic intestinal innervation in aging rats
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DOI：
10.1016/j.nut.2012.09.004
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发表时间：
2013-04-01
期刊：
影响因子：
4.4
通讯作者：
Marcal Natali, Maria Raquel
中科院分区：
文献类型：
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作者：
Cirilo, Carla Possani;Ferreira Schoffen, Joao Paulo;Marcal Natali, Maria Raquel
目的：评价限食对不同年龄大鼠小肠氧化状态、HuC/D-neuronal nitric oxide synthase（nNOS）肌间神经元数量、HuC/D-S100胶质细胞和形态计量学的影响。15只Wistar大鼠分为7月龄和12月龄对照组和12月龄限制饮食实验组（50%的正常日粮）5个月。在7月龄和12月龄时，将动物麻醉，并收集血液以评估生化成分和氧化状态。采用双标记（HuC/D-nNOS和HuC/D-S100）免疫组织化学染色和组织学处理，对肠壁细胞进行形态计量学分析，并测定中期分裂相指数和杯状细胞比率。数据进行方差分析和Tukey事后检验与5%的显着性level.Results：年龄影响的氧化状态，增加脂质过氧化反应，血液成分，内在神经支配，肠壁元素没有影响。进行饮食限制的动物显示总胆固醇、三酰甘油和氧化状态的水平提高，而nNOS神经元群体没有变化。然而，饮食限制显着降低了神经胶质细胞和HuC/D肌间的人口，导致神经元细胞体萎缩，诱导胶质细胞肥大，并减少了肠壁的厚度。结论：高氧化状态的老龄动物逆转饮食限制，这也降低了胆固醇和三酰甘油水平。目前的饮食限制引起回肠肌间神经丛和组织学的形态定量变化，可能对肠道功能产生影响。(C)2013 Elsevier Inc. All rights reserved.
Objectives: To evaluate the effects of dietary restriction on oxidative status, the HuC/D-neuronal nitric oxide synthase (nNOS) myenteric neuron population, HuC/D-S100 glial cells, and the morphometry of the small intestine in rats at various ages.Methods: Fifteen Wistar rats were divided into 7-and 12-mo-old control groups and a 12-mo-old experimental group subjected to dietary restrictions (50% of normal ration) for 5 mo. At 7 and 12 mo of age, the animals were anesthetized, and blood was collected to assess the biochemical components and oxidative status. Ileum samples were subjected to double-marker (HuC/D-nNOS and HuC/D-S100) immunostaining and histologic processing to morphometrically analyze intestinal wall elements and determine the metaphase index and rate of caliciform cells. The data were subjected to analysis of variance and the Tukey post hoc test with a 5% significance level.Results: Age affected the oxidative status by increasing lipid peroxidation, with no effect on blood components, intrinsic innervation, and intestinal wall elements. The animals subjected to dietary restriction showed improved levels of total cholesterol, triacylglycerols, and oxidative status, with no changes in the nNOS neuron population. However, the dietary restriction dramatically decreased the glial and HuC/D myenteric populations, led to atrophy of the neuronal cell body, induced glial hypertrophy, and decreased the thickness of the intestinal wall.Conclusion: The high oxidative status of the aging animals was reversed by dietary restriction, which also lowered cholesterol and triacylglycerol levels. The present dietary restriction elicited morpho-quantitative changes in the myenteric plexus and histology of the ileum, with likely effects on intestinal functions. (C) 2013 Elsevier Inc. All rights reserved.