ANTI-GBM ANTIBODY-INDUCED PROTEINURIA IN ISOLATED PERFUSED RAT-KIDNEY
ANTI-GBM ANTIBODY-INDUCED PROTEINURIA IN ISOLATED PERFUSED RAT-KIDNEY
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DOI：
10.1152/ajprenal.1985.249.2.f241
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
LOWENSTEIN, LM
中科院分区：
文献类型：
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作者：
COUSER, WG;DARBY, C;LOWENSTEIN, LM
The effect os anti-GBM [glomerular basement membrane] antibody on protein excretion was studied in isolated rat kidneys perfused with 20 mg of sheep anti-rat GBM (experimental) or nonantibody sheep IgG (control). Six control kidneys excreted 176 .+-. 31 .mu.g/min of BSA [bovine serum albumin] initially, rising to 296 .+-. 111 .mu.g/min at 2 h. Fractional clearance of BSA rose from 0.51 to 1.70%. Eight experimental kidneys excreted 211 .+-. 56 .mu.g/min of BSA, increasing to 1.924 .+-. 804 .mu.g/min at 2 h. Fractional BSA clearance increased from 0.56 to 11.49%. After 60 min, BSA excretion in anti-GBM-perfused kidneys exceeded controls by a factor of 6.5-7.9 (P < 0.05) and fractional BSA clearance exceeded controls by a factor of 5.8-7.1 (P < 0.05). Studies with fluorescent markers indicated proteinuria to be of glomerula origin in antibody-perfused kidneys. There were no significant differences between anti-GBM-perfused and control kidneys in perfusion pressures, perfusate flow rates, urine flow rates, inulin clearance of Na reabsorption. Antibody to GBM can induce a marked increase in glomerular permeability to BSA and IgG without participation of other systemic humoral or cellular mediation systems.
