Gene expression profiling in the silkworm, Bombyx mori, during early embryonic development
Gene expression profiling in the silkworm, Bombyx mori, during early embryonic development
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DOI：
10.2108/zsj.23.517
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发表时间：
2006-06-01
影响因子：
0.9
通讯作者：
Kang, Seok-Woo
中科院分区：
文献类型：
--
作者：
Hong, Sun-Mee;Nho, Si-Kab;Kang, Seok-Woo
我们准备了一个cDNA文库的微阵列从蚕卵，家蚕mod，在胚带形成（受精后24小时）阶段。使用由2，445个EST构建的微阵列，我们在胚胎早期（从未受精卵到腹足附件形成）的6个特定时间点筛选了生殖带形成过程中的基因表达谱，并确定了其中241个cDNA代表生殖带形成阶段差异表达的基因。这些差异表达基因可分为两类。在早期和晚期簇中，分别有203和38个基因上调。在上调的簇中，我们分离出几个与发育和细胞通讯相关的基因，包括平等主义，RAD23b，innexin 2和衰老相关蛋白。北方杂交结果显示，在各发育阶段有14个基因的表达发生了变化。在这项研究中，我们评估了在整个胚胎胚带形成阶段的基因表达水平的变化。
We prepared a cDNA library for a microarray from eggs of the silkworm, Bombyx mod, at the germ-band formation (24 hours after fertilization) stage. Using a microarray constructed with 2,445 ESTs, we screened gene expression profiles during germ-band formation at six specific time points in the early embryonic stages (from the unfertilized egg to the formation of abdominal leg appendages), and determined 241 of these cDNAs to represent genes that were expressed differentially during the germ-band formation stage. These differentially expressed genes grouped into two clusters. In the early and late clusters, 203 and 38 genes were upregulated, respectively. In the upregulated clusters, we isolated several genes that were associated with development and cell communication, including egalitarian, RAD23b, innexin 2, and senescence-associated protein. Northern blot hybridization revealed that the expression patterns of 14 genes had changed in each of the stages. In this study, we assessed changes in the levels of gene expression in relation to the germ-band formation stages in whole Bombyx embryos.