The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
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DOI：
10.1016/j.jpedsurg.2007.08.039
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发表时间：
2007-12-01
影响因子：
2.4
通讯作者：
Taguchi, Tomoaki
中科院分区：
文献类型：
--
作者：
Hayashida, Makoto;Nishimoto, Yuko;Taguchi, Tomoaki
背景：胆道闭锁是一种病因不明的胆汁淤积性疾病。近年来研究表明，微嵌合体引起的移植物抗宿主病是自身免疫性疾病发生的一个病因。此外，肝脏是移植物抗宿主病的常见靶器官。本研究的目的是确定是否存在和程度的母体微嵌合体，并确定它是否发挥作用的病因BA。方法：6例男性患者BA（BA组）和6例男性与其他肝脏疾病（非BA组）的肝活检标本进行了分析X和Y染色体荧光原位杂交。计数细胞核中含有2条性染色体的细胞。具有1条X染色体和1条Y染色体的细胞被认为是宿主细胞，而具有2条X染色体的细胞被认为是母体来源的。结果：在BA组和非BA组中，每1000个宿主细胞中具有XX染色体的细胞的频率分别为3.00+/-0.75和0.99+/-0.50（P=.005）。此外，在活检时的年龄并没有影响嵌合cells.Conclusion的数量：女性细胞的存在下，在男性患者的肝脏BA显着高于男性与其他肝脏疾病。因此，母亲的微嵌合体的BA的发病机制作出贡献。(C)2007爱思唯尔公司All rights reserved.
Background: Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA.Methods: The liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin.Results: The frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non BA group were 3.00+/-0.75 and 0.99+/-0.50, respectively (P=.005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells.Conclusion: The presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA. (C) 2007 Elsevier Inc. All rights reserved.