An initial characterization of the 3′ untranslated region of the EhPgp5 mRNA in Entamoeba histolytica
An initial characterization of the 3′ untranslated region of the EhPgp5 mRNA in Entamoeba histolytica
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DOI：
10.1016/s0188-4409(00)00174-0
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发表时间：
2000-07-01
影响因子：
7.7
通讯作者：
Orozco, E
中科院分区：
文献类型：
--
作者：
López, C;Marchat, LA;Orozco, E
在溶组织内阿米巴中，EhPgp1和EhPgp5基因的过表达导致了多药耐药表型（MDR）。转录调控在这些基因表达的机制中起着重要作用（1）。然而，翻译后事件的相关性尚未得到探讨。在培养的大鼠肝细胞中，Pgp 2基因过表达主要是由于转录后机制，其中mRNA稳定性的变化似乎是关键的（2）。此外，在大鼠肝肿瘤中，Pgp 1、Pgp 2和Pgp 3基因的过表达似乎是由涉及mRNA稳定的机制引起的（3）。3个非翻译区（3 UTR）序列影响mRNA的稳定性及其多聚腺苷酸化和翻译，核输出和效率加工。周转率和RNA切割率的变化受3个UTR二级结构基序的形成和与这些结构结合的蛋白质的影响。E.溶组织菌EhPgp基因也可能受RNA结构的调控。作为研究EhPgp5基因表达的转录后机制的第一步，我们分析了EhPgp5 mRNA的3个非翻译区的相关序列特征和二级结构。
In Entamoeba histolytica, the multidrug resistance phenotype (MDR) is given by the overexpression of the EhPgp1 and EhPgp5 genes. Transcriptional regulation plays an important role in the mechanisms underlying the expression of these genes (1). However, the relevance of postranscriptional events has not yet been explored. In cultured rat hepatocytes, Pgp2 gene overexpression is primarily due to postranscriptional mechanisms, in which changes in mRNA stability appear to be critical (2). In addition, in rat liver tumors the overexpression of the Pgp1, Pgp2, and Pgp3 genes seems to be caused by a mechanism involving mRNA stabilization (3). The 3 untranslated region (3 UTR) sequence affects mRNA stability and its polyadenylation and translation, nuclear export, and efficiency processing. Changes in rates of turnover and RNA cleavage are affected by the formation of 3 UTR secondary structure motifs and by proteins binding to these structures. Postranscriptional events of E. histolytica EhPgp genes may be also regulated by RNA structure. As an initial step to study the postranscriptional mechanisms involved in EhPgp5 gene expression, we analyze here the relevant sequence features and the secondary structure of the 3 UTR of EhPgp5 mRNA.