Accumulation of oxidized LDL in human semilunar valves correlates with coronary atherosclerosis
Accumulation of oxidized LDL in human semilunar valves correlates with coronary atherosclerosis
复制标题
DOI：
10.1016/s0008-6363(99)00389-2
复制
发表时间：
2000-03-01
影响因子：
10.8
通讯作者：
Lang, IM
中科院分区：
文献类型：
--
作者：
Mehrabi, MR;Sinzinger, H;Lang, IM
目的：最近的研究表明，氧化低密度脂蛋白（ox-LDL）具有多种促动脉粥样硬化作用，如诱导巨噬细胞趋化剂、粘附分子、细胞因子、1型纤溶酶原激活物抑制剂和血小板源性生长因子a链等。因此，ox-LDL已被用作动脉粥样硬化中蛋白质氧化修饰的标记物。由于心脏瓣膜由平滑肌细胞、成纤维细胞和内皮细胞组成，并且由于瓣膜疾病和冠状动脉粥样硬化可能由相似的生物学过程引起，我们研究了血管造影证实的冠心病（CHD, n=19）、特发性充血性心力衰竭（IDCM=特发性扩张型心肌病，n=20）患者和移植供者的离体主动脉瓣、肺动脉瓣和冠状动脉中ox-LDL的积累。方法：对新鲜离体半月瓣石蜡切片进行Masson-Goldner染色和抗ox-LDL、CD68免疫组化。采用数字图像平面测量法和视觉染色强度评分法对数据进行分析。结果：Ox-LDL免疫反应性在附着线血管面、深瓣间质、半月瓣心室和血管内皮中被发现，并与巨噬细胞共定位。冠心病患者的瓣膜ox-LDL面积明显增加(P
Objective: Recent data indicate that oxidized low-density lipoprotein (ox-LDL) has several proatherogenic effects, e.g. induction of macrophage chemoattractants, adhesion molecules, cytokines, type-1 plasminogen activator inhibitor and platelet-derived growth factor A-chain by smooth muscle cells. Therefore, ox-LDL has been utilized as a marker of oxidative modification of proteins in atherosclerosis. Because heart valves consist of smooth muscle cells, fibroblasts and endothelial cells, and because valvular disease and coronary atherosclerosis could result from similar biological processes, we investigated ox-LDL accumulation in isolated aortic and pulmonary valves and coronary arteries from patients with angiographically proven coronary heart disease (CHD, n=19), patients with idiopathic congestive heart failure (IDCM=idiopathic dilated cardiomyopathy, n=20), and transplant donors. Methods: Masson-Goldner staining and immunohistochemistry utilizing anti ox-LDL and CD68 were performed on paraffin sections of freshly isolated semilunar valves. Data were analyzed by digital image planimetry and by visual scoring of staining intensity. Results: Ox-LDL immunoreactivity was identified in the vascular aspect of the attachment line, in the deep valve stroma, and in the ventricular and vascular endothelium of the semilunar valves, colocalizing with macrophages. Valvular ox-LDL area was significantly increased in CHD-patients (P