Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis
Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis
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DOI：
10.4161/15592324.2014.989050
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发表时间：
2015-02
影响因子：
2.9
通讯作者：
H. Kaya;M. Iwano;S. Takeda;M. Kanaoka;Sachie Kimura;Mitsutomo Abe;K. Kuchitsu
中科院分区：
文献类型：
--
作者：
H. Kaya;M. Iwano;S. Takeda;M. Kanaoka;Sachie Kimura;Mitsutomo Abe;K. Kuchitsu
活性氧(ROS)积累在正在生长的花粉管的顶端。在拟南芥中，NADPH氧化酶RbohH和RbohJ定位于花粉管顶端的质膜上，以钙离子依赖的方式产生ROS。Rbohs和Ca~(2+)产生的ROS可能在维持顶端生长的正反馈调节中起关键作用。超微结构细胞化学分析表明，野生型ROS在柱头乳突上的质外体/细胞壁中积累，而rbohH/rbohJ双突变体不积累，表明RbohH和RbohJ的质外体ROS通过影响细胞壁代谢参与了花粉管向柱头乳突的伸长。
Reactive oxygen species (ROS) accumulate at the tip of growing pollen tubes. In Arabidopsis, NADPH oxidases RbohH and RbohJ are localized at the plasma membrane of pollen tube tip and produce ROS in a Ca2+-dependent manner. The ROS produced by Rbohs and Ca2+ presumably play a critical role in the positive feedback regulation that maintains the tip growth. Ultrastructural cytochemical analysis revealed ROS accumulation in the apoplast/cell wall of the pollen grains on the stigmatic papillae in the wild type, but not in the rbohH rbohJ double mutant, suggesting that apoplastic ROS derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism.