Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells
Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells
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DOI：
10.1002/jbm.a.34774
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发表时间：
2014-04-01
影响因子：
4.9
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;Huang, Yan;Fan, Yubo
结合使用细胞和材料的联合收割机的再生医学治疗可能为组织/器官修复和再生开辟新的选择。骨髓间充质干细胞（mesenchymal stem cells，MSCs）的微环境严格调控其自我更新和功能。本研究将第2-4代大鼠骨髓间充质干细胞（rBMSCs）和脂肪间充质干细胞（rAMSCs）分别培养于不同的基质上，发现它们的细胞功能表现出对基质硬度的依赖性。细胞在较软的基质上的贴壁情况比在较硬的基质上好。基底硬度对细胞增殖无明显影响。但基质硬度显著促进两种干细胞的成骨分化。此外，在相同刚度下培养的rBMSCs比rAMSCs表达更多的成骨细胞相关标志物。此外，生物材料和生化试剂的组合处理产生了更强的影响，骨髓间充质干细胞的成骨分化比单独处理。这些结果对进一步扩展我们在工程功能组织替代物方面的能力具有重要意义。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102 A：1092-1101，2014。
Regenerative medicine treatments that combine the use of cells and materials may open new options for tissue/organ repair and regeneration. The microenvironment of mesenchymal stem cells (MSCs) strictly regulates their self-renewal and functions. In this study, when rat bone marrow derived MSCs (rBMSCs) and rat adipose tissue derived MSCs (rAMSCs) in passages 2-4 were cultured on different substrates, they presented the cellular functions to be dependent of substrate stiffness. The cells attached better on the softer substrate than on the stiffer one. The substrate stiffness had no significant influence on the proliferation of those cells. However, the substrate stiffness significantly promoted the osteogenic differentiation of the two kinds of stem cells. Furthermore, rBMSCs cultured on the same stiffness expressed more osteoblast-related markers than rAMSCs. In addition, combined biomaterials and biochemical reagents treatment yielded a stronger effect on osteogenic differentiation of MSCs than either treatment alone. These results have significant implications for further extending our capabilities in engineering functional tissue substitutes. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1092-1101, 2014.