Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets
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DOI：
10.1002/1873-3468.12901
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发表时间：
2017-12
期刊：
影响因子：
3.5
通讯作者：
Norito Tamura;Taki Nishimura;Y. Sakamaki;Ikuko Koyama-Honda;Hayashi Yamamoto;N. Mizushima
中科院分区：
文献类型：
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作者：
Norito Tamura;Taki Nishimura;Y. Sakamaki;Ikuko Koyama-Honda;Hayashi Yamamoto;N. Mizushima
ATG 2是自噬体形成所必需的自噬相关（ATG）蛋白之一，并定位于哺乳动物细胞中的隔离膜和脂滴。在这里，我们调查的区域在ATG 2A的细胞器定位和功能的要求。N端氨基酸1-198和C端氨基酸1830-1938分别是定位于分离膜和脂滴所需的。C-末端区域不需要定位到隔离膜和自噬。我们还鉴定了ATG 2A中的两亲性螺旋，这是其定位于细胞器和自噬体形成所必需的。这些数据表明，ATG 2A的双重定位是由不同的区域调节。
ATG2 is one of the autophagy‐related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N‐terminal amino acids 1–198 and the C‐terminal amino acids 1830–1938 are required for the localization to isolation membranes and lipid droplets, respectively. The C‐terminal region is not required for the localization to isolation membranes and for autophagy. We also identified an amphipathic helix in ATG2A that is required for both its localization to organelles and autophagosome formation. These data suggest that the dual localization of ATG2A is regulated by different regions.