Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities
Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities
复制标题
DOI：
10.2136/sssaj2000.6451659x
复制
发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Dick, RP
中科院分区：
文献类型：
--
作者：
Schutter, ME;Dick, RP
脂肪酸谱是表征自然系统微生物群落的常用方法。与首先提取脂质然后从脂质中释放脂肪酸的方法相比，原位直接提取微生物脂肪酸将是有用的。本研究以三种耕作粉壤土和一种林地桑迪粘壤土为研究对象，比较了两种直接提取土壤脂肪酸的方法。通过酯连接（EL）方法和微生物鉴定方法（Microbial ID，Inc.，纽瓦克，DE）。土壤以四种不同的方式储存（4 ℃潮湿、-20 ℃潮湿、25 ℃风干和4 ℃部分干燥），并在储存30和90 d后分析FAME分布的变化。11和17 FAME是独特的EL和EFL方法，分别，但独特的FAME一般被发现只有微量。土壤提取的方法产生更多的羟基FAME和短链饱和和支化FAME。相反，EL提取的土壤一般产生更多的长链饱和和支化FAME，不饱和FAME，和FAME与环丙烷和甲基。这两种提取方法都能够区分不同土壤类型的社区，无论土壤是新鲜的还是储存的。FAME配置文件的变化确实发生在存储的土壤，但每个存储协议的有效性，随着时间的推移保持FAME模式是不同的四种土壤。虽然群落分析应在新鲜土壤中进行，但与提取方法和土壤类型相比，储存的总体影响较小。
Fatty acid profiling is a popular method for characterizing microbial communities of natural systems. Direct extraction of microbial fatty acids in situ would be useful compared with methods that extract lipids first and subsequently release fatty acids from lipids. In this study, two methods fur the direct extraction of fatty acids from soils were compared for three cultivated silt loams and one forested sandy clay loam. Fresh soils were analyzed for their fatty acid methyl ester (FAME) profiles by an ester-linked (EL) method and the method of MIDI (Microbial ID, Inc., Newark, DE). Soils were stored four different ways (moist at 4 degrees C, moist at -20 degrees C, air-dried at 25 degrees C, and partially dry at 4 degrees C) and analyzed for FAME profile changes after 30 and 90 d of storage. Eleven and 17 FAMEs were unique to the EL and MIDI method, respectively, but unique FAMEs generally were found in only minute amounts. Soils extracted with the MIDI method yielded more hydroxy FAMEs and short-chain saturated and branched FAMEs. Conversely, EL-extracted soils generally produced more long-chain saturated and branched FAMEs, unsaturated FAMEs, and FAMEs with cyclopropane and methyl groups. Both extraction methods were able to differentiate among communities of different soil types, regardless if soils were fresh or stored. Changes in FAME profiles did occur in stored soils, but the effectiveness of each storage protocol for preserving FAME patterns over time was different among the four soils. While community analyses should be conducted on fresh soil, overall effects of storage were slight compared with those of extraction method and soil type.