Monophyletic origin of naked barley inferred from molecular analyses of a marker closely linked to the naked caryopsis gene (nud)
Monophyletic origin of naked barley inferred from molecular analyses of a marker closely linked to the naked caryopsis gene (nud)
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DOI：
10.1007/s00122-003-1560-1
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Kawasaki, S
中科院分区：
文献类型：
--
作者：
Taketa, S;Kikuchi, S;Kawasaki, S
为了阐明裸大麦的起源，研究了与nud位点紧密连锁的标记sKT 7的分子变异。共259（53野生，106带壳驯化，100裸驯化）大麦种质进行了研究。sKT 7 PCR扩增产物的限制性分析显示等位基因I、II、III和IV。所有四个等位基因被发现在野生大麦，但等位基因IV被发现只在一个单一的加入从伊朗西南部。带壳的驯化加入显示等位基因I，II，或III，但所有裸驯化加入等位基因IV。等位基因IV在野生大麦中的分布及其在裸大麦驯化品系中的普遍存在支持裸大麦可能在伊朗西南部具有单系起源的结论。现有结果表明裸大麦的起源有两种情况：要么直接来自具有等位基因IV的野生大麦，要么来自具有等位基因IV的带壳驯化品系，但后来灭绝了。来自世界不同地区的裸驯化种质在sKT 7基因座处具有非常均一的DNA序列，支持裸大麦的单系起源。对于等位基因IV，4个单倍型（IVb至IVe）被发现在30裸加入：IVb是占主导地位的（66.7%）和广泛分布，而其他三个单倍型，不同的只有一个核苷酸在不同的位置相对于IVb，显示出局部分布。sKT 7等位基因IV单倍型的地理分布表明裸驯化大麦在中亚和东亚的迁移路线。
To elucidate the origin of naked barley, molecular variation of the marker sKT7 tightly linked to the nud locus was examined. A total of 259 (53 wild, 106 hulled domesticated, and 100 naked domesticated) barley accessions were studied. Restriction analysis of the sKT7 PCR-amplified product revealed the alleles I, II, III, and IV. All four alleles were found in wild barley, but allele IV was found only in a single accession from southwestern Iran. Hulled domesticated accessions showed alleles I, II, or III, but all naked domesticated accessions had allele IV. The distribution of allele IV in wild barley and its pervasive presence in naked domesticated lines support the conclusion that naked barley has a monophyletic origin, probably in southwestern Iran. The available results suggest two scenarios for the origin of naked barley: either directly from a wild barley with allele IV or from a hulled domesticated line with allele IV that later became extinct. Naked domesticated accessions from different regions of the world have extremely homogeneous DNA sequences at the sKT7 locus, supporting the monophyletic origin of naked barley. For allele IV, four haplotypes (IVb to IVe) were found in 30 naked accessions: IVb was predominant (66.7%) and widely distributed, while the other three haplotypes, differing by only one nucleotide at different positions relative to IVb, showed a localized distribution. The geographical distribution of the haplotypes of sKT7 allele IV suggests migration routes of naked domesticated barley in central and eastern Asia.