Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique.
Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique.
复制标题
阴道毛滴虫分离株的地理变异：通过使用单克隆抗体和间接免疫荧光技术证明抗原异质性。
DOI：
10.1093/infdis/152.5.979
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Tam,MR
中科院分区：
文献类型：
--
作者：
Krieger,JN;Holmes,KK;Spence,MR;Rein,MF;McCormack,WM;Tam,MR
尽管阴道毛滴虫是最常见的性传播疾病之一，但对寄生虫的抗原变异或流行病学或毒力菌株之间的差异知之甚少。菌株间的变异。通过使用一组单克隆抗体对来自北美不同地理区域的88个分离株进行检测，每个单克隆抗体与不同的抗原反应。所有菌株均为T.阴道分泌物与9种单克隆抗体中的至少一种发生反应，单个抗体与22%~ 76%的分离株发生反应。两种具有广泛反应性的抗体池鉴定了研究中的所有分离株。四种最窄反应性或“特异性”的抗体显示了从西雅图、巴尔的摩和纽约布鲁克林患者中分离的滴虫的抗原组成的差异（χ 2检验P<0.005）。这些单克隆抗体探针的应用可能有助于流行病学研究，并为直接诊断临床标本中的滴虫提供快速，可靠的方法。
AlthoughTrichomonas vaginaliscauses one of the most common sexually transmitted diseases, little is known about the antigenic variation of the parasite or about differences between strains in epidemiology or virulence. Variation among isolates ofT. vaginaliswas investigated by using a panel of monoclonal antibodies, each reactive with different antigens, to test 88 isolates from diverse geographic areas of North America. All isolates ofT. vaginalisreacted with at least one of the nine monoclonal antibodies; the individual antibodies reacted with 22%–76% of the isolates. A pool of two broadly reactive antibodies identified all isolates in the study. Four of the most narrowly reactive, or “specific,” antibodies demonstrated differences in the antigenic composition of trichomonads isolated from patients in Seattle, Baltimore, and Brooklyn, New York (P< .005 by χ2test). Application of these and other monoclonal antibody probes may facilitate epidemiological studies and provide rapid, reliable methods for direct diagnosis of trichomonads in clinical specimens.