GhHyPRP4, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress
GhHyPRP4, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress
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GhHyPRP4 是一种编码假定的杂合富含脯氨酸蛋白的棉花基因，优先在叶子中表达并参与植物对冷胁迫的反应
DOI：
10.1093/abbs/gmr040
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发表时间：
2011-07-01
影响因子：
3.7
通讯作者：
Li, Xuebao
中科院分区：
文献类型：
--
作者：
Huang, Gengqing;Gong, Siying;Li, Xuebao
植物杂交富含脯氨酸蛋白(HyPRPs)通常由一个N-端信号肽、一个中央富含Pro结构域和一个保守的8-半胱氨酸基序C-末端结构域组成。在本研究中，从棉花cDNA文库中分离到一个编码HyPRP的基因(命名为GhHyPRP4)。Northern杂交和定量逆转录聚合酶链式反应分析表明，GhHyPRP4在叶片中优先表达。在低温胁迫下，GhHyPRP4在棉苗叶片中的表达显著上调。利用基因组步行的方法，从棉花基因组中分离到GhHyPRP4基因的启动子片段。GhHyPRP4启动子驱动的GUS(β-葡萄糖醛酸酶)基因在转基因拟南芥的叶片和子叶中特异表达。此外，冷胁迫显著诱导叶片中GUS的表达。GhHyPRP4在裂殖酵母中的过表达显著提高了棉花幼苗在-20℃下处理60h后的细胞存活率，这些结果表明GhHyPRP4可能参与了棉花幼苗发育过程中植物对低温胁迫的反应。
Plant hybrid proline-rich proteins (HyPRPs) usually consist of an N-terminal signal peptide, a central proline-rich domain, and a conserved eight-cysteine motif C-terminal domain. In this study, one gene (designated as GhHyPRP4) encoding putative HyPRP was isolated from cotton cDNA library. Northern blot and quantitative reverse transcriptase-polymerase chain reaction analyses revealed that GhHyPRP4 was preferentially expressed in leaves. Under cold stress, GhHyPRP4 expression was significantly up-regulated in leaves of cotton seedlings. Using the genome walking approach, a promoter fragment of GhHyPRP4 gene was isolated from cotton genome. GUS (beta-glucuronidase) gene driven by GhHyPRP4 promoter was specifically expressed in leaves and cotyledons of the transgenic Arabidopsis thaliana. Furthermore, GUS expression in leaves was remarkably induced by cold stress. Overexpression of GhHyPRP4 in yeast (Schizosaccharomyces pombe) significantly enhanced the cell survival rate upon treatment under -20 degrees C for 60 h. These data suggested that GhHyPRP4 may be involved in plant response to cold stress during seedling development of cotton.