Expression of COUP-TFII in metabolic tissues during development
Expression of COUP-TFII in metabolic tissues during development
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DOI：
10.1016/s0925-4773(02)00286-1
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发表时间：
2002-11-01
影响因子：
2.6
通讯作者：
Vasseur-Cognet, M
中科院分区：
文献类型：
--
作者：
Zhang, P;Bennoun, M;Vasseur-Cognet, M
在哺乳动物中，COUP-TF家族由两种结构相关的蛋白质COUP-TFI和COUP-TFII组成，属于类固醇/甲状腺激素受体超家族的孤儿成员。为了深入了解COUP-TFII的作用，我们研究了小鼠COUP-TFII的发育表达模式，重点研究了内胚层来源的组织，特别是胰腺和肝脏。产生表达由COUP-TFII启动子驱动的大肠杆菌β-半乳糖苷酶的转基因小鼠的独立系。胚胎表达的β-半乳糖蛋白在妊娠第9天检测到脊索，腹侧神经管，有趣的是，在肠道内胚层，一个网站COUP-TFII尚未被检测到。在9.5和11.5 dpc之间，早期建立的β-gal表达模式持续存在，切片显示心脏的共同心房腔染色。在15.5 dpc时，在所有内胚层来源的组织中发现β-gal活性。我们发现，COUP-TFII mRNA和蛋白存在于胎儿和成人肝细胞。最后，在胰腺中检测到COUP-TFII表达，如通过在12.5dpc时在一些胰高血糖素和PDX 1阳性细胞中β-gal的共表达以及在15.5dpc时与胰岛素阳性细胞的共表达所判断的。在成人胰腺，COUP-TFII蛋白存在于内分泌胰岛细胞。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
In mammals, the COUP-TF-family consisting of two structurally related proteins, COUP-TFI and COUP-TFII belongs to the orphan member of the steroid/thyroid hormone receptor superfamily. In an attempt to gain insights into the role of COUP-TFII, we examined developmental expression pattern of the mouse COUP-TFII focusing our studies on endoderm-derived tissues, pancreas and liver in particular. Independent lines of transgenic mice expressing Escherichia coli beta-galactosidase driven by the COUP-TFII promoter were generated. Embryonic expression of the beta-gal protein at day 9 of gestation was detected in the notochord, the ventral neural tube and, interestingly, in the gut endoderm, a site where COUP-TFII has not been detected previously. Between 9.5 and 11.5 dpc, beta-gal expression pattern that was established earlier persisted and sections revealed a staining of the common atrial chamber of the heart. At 15.5 dpc, beta-gal activity was found in all endoderm-derived tissues. We found that COUP-TFII mRNA and protein were present in fetal and adult hepatocytes. Finally, COUP-TFII expression was detected in pancreas, as judged by co-expression of the beta-gal in some of the glucagon and PDX1 positive-cells at 12.5 dpc and co-expression with insulin positive-cells at 15.5 dpc. In adult pancreas, COUP-TFII protein was present in the endocrine islet cells. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.