Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex
Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex
复制标题
DOI：
10.1016/s1097-2765(00)80409-8
复制
发表时间：
2000-01-01
期刊：
影响因子：
16
通讯作者：
Silver, PA
中科院分区：
文献类型：
--
作者：
Damelin, M;Silver, PA
The interactions between transport receptors and proteins of the nuclear pore complex (NPC) are fundamental to understanding nucleocytoplasmic transport. In order to delineate the path that a particular transport receptor takes through the NPC, we have employed fluorescence resonance energy transfer (FRET) between enhanced cyan and yellow fluorescent proteins (ECFP, EYFP) in living cells. A panel of yeast strains expressing functional receptor-ECFP and nucleoporin-EYFP fusions has been analyzed with a FRET assay. With this approach, we define points of contact in the NPC for the related importin Pse1/Kap121 and exportin Msn5. These data demonstrate the utility of FRET in mapping dynamic protein interactions in a genetic system. Furthermore, the data indicate that an importin and exportin have overlapping pathways through the NPC.
