Differences in the transient response of fast and slow skeletal muscle fibers. Correlations between complex modulus and myosin light chains.
Differences in the transient response of fast and slow skeletal muscle fibers. Correlations between complex modulus and myosin light chains.
复制标题
快速和慢速骨骼肌纤维瞬态响应的差异。
DOI：
10.1016/s0006-3495(84)84262-9
复制
发表时间：
1984
影响因子：
3.4
通讯作者：
Schachat,FH
中科院分区：
文献类型：
--
作者：
Kawai,M;Schachat,FH
对几种兔骨骼肌(腰大肌、胫前肌、指长伸肌、横隔肌、比目鱼肌、半腱肌)的肌纤维在最大激活状态下的机械力化学性质进行了正弦分析。这项研究区分了两大类纤维，根据它们的肌球蛋白轻链补体，可以分为快纤维和慢纤维。在快速纤维(例如腰大肌)中，我们发现至少存在三个大小相当的指数过程(A)、(B)、(C)。在慢纤维(例如比目鱼肌)中，我们发现至少存在四个大小不同的指数过程(A)-(D)；与(C)和(D)相比，(A)和(B)的大小非常小。在慢纤维中，表观速率常数慢8-29倍。由于我们的正弦表征所用时间小于或等于22 S，并且不涉及化学变性或其他破坏肌丝晶格的方法，因此它允许表征不同生理类别的纤维，然后用其他技术进一步研究。正弦分析后用凝胶电泳法测定的生理和分子特性之间的完美相关性证明了这一点，并证明了它在区分纤维类型方面的用途。
Sinusoidal analysis of the mechanochemical properties of skinned muscle fibers under conditions of maximal activation was applied to fibers from several rabbit skeletal muscles (psoas, tibialis anterior, extensor digitorum longus, diaphragm, soleus, semitendinosus). This investigation distinguished between two general classes of fibers, which on the basis of their myosin light chain complements could be classified as fast and slow. In fast fibers (e.g., psoas) we identified the presence of at least three exponential processes (A), (B), (C) of comparable magnitudes. In slow fibers (e.g., soleus) we identified the presence of at least four exponential processes (A)-(D) of very different magnitudes; magnitudes of processes (A) and (B) are very small compared with those of (C) and (D). The apparent rate constants are 8–29-fold slower in slow fibers. Because our sinusoidal characterization takes less than or equal to 22 s and does not involve chemical denaturation or other means of disruption of the myofilament lattice, it allows the different physiological classes of fibers to be characterized and then studied further by other techniques. The perfect correlation between physiological and molecular properties as assayed by gel electrophoresis after sinusoidal analysis demonstrates this and justifies its use in distinguishing between fiber types.