Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid γ-Lactone Chemiluminescence
Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid γ-Lactone Chemiluminescence
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DOI：
10.2116/analsci.21.701
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发表时间：
2005-06
影响因子：
1.6
通讯作者：
T. Kamidate;Nobuhito Kikuchi;A. Ishida;H. Tani
中科院分区：
文献类型：
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作者：
T. Kamidate;Nobuhito Kikuchi;A. Ishida;H. Tani
应用均质龙胆酸γ-内酯（HAL）化学发光（CL）测定脂质体中辣根过氧化物酶（HRP）的含量。用 Triton X-100 裂解 HRP 捕获的脂质体后检测到 HRP。将 HAL CL 中 HRP 的 CL 响应率、检出限和校准曲线线性范围与对碘苯酚 (p-IP) 增强鲁米诺 CL 中的进行比较。与 p-IP 增强鲁米诺 CL 相比，HAL CL 中的最大光发射出现得更快，从而导致 CL 测量时间显着缩短。 HAL CL 中 HRP 的检测限与 p-IP 增强鲁米诺 CL 中的检测限相同。与 p-IP 增强鲁米诺 CL 相比，HAL CL 中 HRP 校准曲线的线性范围提高了 50 倍。从这些结果可以看出，在测定脂质体中封装的 HRP 方面，HAL CL 优于 p-IP 增强的鲁米诺 CL。
Homogentisic acid γ -lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p -iodophenol ( p -IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p -IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p -IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p -IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p -IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.