HETEROGENEITY OF IMMUNOGLOBULIN GENE REARRANGEMENTS IN B-CELL LYMPHOMAS
HETEROGENEITY OF IMMUNOGLOBULIN GENE REARRANGEMENTS IN B-CELL LYMPHOMAS
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DOI：
10.1002/ijc.2910450406
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发表时间：
1990-04-15
影响因子：
6.4
通讯作者：
KRIEGER, G
中科院分区：
文献类型：
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作者：
KNEBA, M;BERGHOLZ, M;KRIEGER, G
我们对69例B细胞性非霍奇金S淋巴瘤进行了免疫球蛋白(Ig)或T细胞抗原受体(TcR)基因重排检测。淋巴瘤被归入基尔分类，并通过免疫组织化学染色证实其B细胞性质。仅2例(CLL)出现克隆的Tβ链TCR基因重排及重链和轻链Ig基因重排。其余67例淋巴瘤具有胚系β链TCR基因配置。鉴定出三种不同的免疫球蛋白基因重排模式：(A)同时存在重链和轻链重排(H+L+)；(B)仅存在重链基因重排(H+L-)；(C)胚系构型的重链和轻链基因(H-L-)。45例低度恶性NHL和4例免疫母细胞性淋巴瘤均为A型，均有kappa基因重排或缺失。在接受检测的24例低度恶性淋巴瘤中，有13例(54%)有lambda轻链基因的额外重排。19例高级别中心母细胞(CB)B-NHL具有不同的Ig基因重排类型：A型12例，B型4例，C型2例。在这组病例中，17例中仅2例(12%)有lambda轻链重排，18例中有12例(67%)有kappa基因重排或缺失。在1例表达Sigm/lambda并有重链Ig重排的病例中，没有DNA可用于Ig轻链分析。
We have examined 69 B-cell non-Hodgkin''s lymphomas (NHL) for rearrangements of the immunoglobulin (Ig) or T-cell antigen receptor (TCR) genes. The lymphomas were assigned to the categories of the Kiel classification and their B-cell nature was confirmed by immunostaining. Only 2 cases (with CLL) displayed cloned T.beta.-chain TCR gene rearrangements together with rearranged heavy- and light-chain Ig genes. The remaining 67 lymphomas had a germline .beta.-chain TCR-gene configuration. Three different patterns of Ig gene rearrangements were identified; (A) presence of both heavy- and light-chain rearrangements (H+L+); (B) rearrangement of heavy-chain gene only (H+L-); (C) heavy- and light-chain genes in germline configuration (H-L-). All the 45 low-grade NHLs and the 4 immunoblastic lymphomas exhibited pattern A and all had their kappa gene rearranged or deleted. Of 24 low-grade lymphomas tested, 13 (54%) had an additional rearrangement of the lambda light-chain gene. In contrast, the 19 high-grade centroblastic (cb) B-NHLs had distinct patterns of Ig-gene rearrangement: 12 with pattern A, 4 with B and 2 with C. In this group only 2 of 17 (12%) cases analyzed had evidence of lambda light-chain rearrangement whereas 12 of 18 (67%) had a kappa gene rearrangement or deletion. In one case expressing sIgM/lambda and with heavy chain Ig-rearrangement, no DNA was available for Ig light-chain analysis.