Stable cavitation using acoustic phase-change dodecafluoropentane nanoparticles for coronary micro-circulation thrombolysis.
Stable cavitation using acoustic phase-change dodecafluoropentane nanoparticles for coronary micro-circulation thrombolysis.
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DOI：
10.1016/j.ijcard.2018.06.027
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发表时间：
2018-12
影响因子：
3.5
通讯作者：
Bo Hu;N. Jiang;Qing Zhou;S. Cao;Shunji Gao;Binghong Zhang;Jin‐ling Chen;R. Guo
中科院分区：
文献类型：
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作者：
Bo Hu;N. Jiang;Qing Zhou;S. Cao;Shunji Gao;Binghong Zhang;Jin‐ling Chen;R. Guo
背景急性心肌梗死后的微循环溶栓治疗一直是一个尚未解决的问题，因为急性溶栓治疗和早期介入治疗的效果并不理想。使用声学相变纳米颗粒的稳定空化可能具有溶栓的潜力。因此，我们试图探索一种新的治疗方法，利用十二氟戊烷纳米粒子(DDFP)在体外人工血管系统中快速有效地溶栓，作为模拟冠状动脉循环的准备。方法建立具有空化效应的DDFP纳米粒子体外人工血管系统，模拟冠状动脉循环中的血栓栓塞。PBS空白对照组(A组)、SonoVue微泡(B组)和DDFP纳米粒(C组)，结果声空化效果的最佳条件为超声功率6 W，时间20 ，温度37 °C。C组血栓失重率和失重率(189.4 ± 30.2 mg和34.2 ± 5.7%)均高于A组(30.2 ± 16.0 mg和34.2%± 5.7%)，C组血栓失重率和失重率均高于A组(P<0.05)。5.2 ± 2.1%)和B组(84.0 ± 2 0.4 mg和14.6 ± 1.5%)(P <； 0.01，全部)。C组空化作用持续时间(32.8 ± 3.9 m in)也明显长于A组(0.0 ± 0.0 m in)和B组(5.3 ± 0.3 m in)(P <； 0.0 1，均P<0.0 1)。
BackgroundThe thrombolysis in micro-circulation after acute myocardial infarction has been an unsolved issue, as elimination effect of acute thrombolysis and primary intervention were unsatisfied. Stable cavitation using acoustic phase-change nanoparticles may have potential for thrombolysis. Therefore, we sought to investigate a novel treatment method with dodecafluoropentane (DDFP) nanoparticles for rapid and effective thrombolysis in an in-vitro artificial vascular system, as a mimicking preparation of coronary circulation.MethodsTo simulate thrombus embolism in coronary circulation, an in-vitro artificial vascular system was established with cavitation effect using DDFP nanoparticles. For PBS blank control (group A), SonoVue microbubbles (group B) and DDFP nanoparticles (group C), the durations for cavitation effect were recorded and the thrombolysis efficiency with low intensity focused ultrasound irradiation in the in-vitro vascular system were analyzed with weight loss and pathological changes of thrombus before and after thrombolysis.ResultsThe optimal conditions for acoustic cavitation effect were power of 6 W for 20 min by ultrasound irradiation at 37 °C. The weight loss and weight loss rates of thrombus in group C (189.4 ± 30.2 mg and 34.2 ± 5.7%) were higher than those in group A (30.2 ± 16.0 mg and 5.2 ± 2.1%) and group B (84.0 ± 20.4 mg and 14.6 ± 1.5%) (P < 0.01, all). The duration for cavitation effect in group C (32.8 ± 3.9 min) was also longer than those in group A (0.0 ± 0.0 min) and group B (5.3 ± 0.3 min) (P < 0.01, all).ConclusionsBy stable and sustaining cavitation in targeted area, DDFP nanoparticles with ultrasound irradiation have significantly increased the thrombolysis efficiency, which has provided a powerful experimental foundation for potential coronary thrombolysis.